Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria
| Autor: | Dolores Peruč, Sanja Štifter, Maja Abram, Mladenka Malenica Staver, Ivana Gobin, Dalibor Broznić, Brigita Tićac |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) nontuberculous mycobacteria medicine.drug_class 030106 microbiology checkerboard synergy method Microbial Sensitivity Tests Antimycobacterial Microbiology Helichrysum italicum Cell wall HeLa 03 medical and health sciences BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Medical Microbiology medicine Oils Volatile Humans Plant Oils essential oils Helichrysum biology Chemistry antibiofilm BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences antiadhesion nontuberculous mycobacteria opportunistic premise plumbing pathogens Biofilm opportunistic premise plumbing pathogens Drug Synergism Nontuberculous Mycobacteria General Medicine biology.organism_classification Anti-Bacterial Agents 030104 developmental biology Biofilms Juniperus Juniperus communis Nontuberculous mycobacteria BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Medicinska mikrobiologija Mycobacterium HeLa Cells |
| Zdroj: | Journal of Medical Microbiology Volume 68 Issue 5 |
| ISSN: | 0022-2615 |
| Popis: | Objective. The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis (J. communis) and Helichrysum italicum (H. italicum) essential oils (EO). Methods. Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checkerboard synergy method. Antiadhesion and antibiofilm effect of EOs was evaluated on biotic (HeLa cells) and abiotic surface (polystyrene). To evaluate the possible mechanisms of action, cellular leakage of proteins and DNA was tested and structural changes were visualized with a transmission electron microscope. Results. MIC, MBC and MEC were 1.6 mg/ml for J. communis EO and 3.2 mg/ml for H. italicum EO against both mycobacteria. All combinations of EOs in checkerboard synergy method produced fractional inhibitory concentration index values ranging from 0.501 to 1.5, corresponding to synergistic, additive or indifferent effects. Mycobacterium avium showed a greater tendency to create biofilm but these EO at subinhibitory concentrations (sMIC) effectively blocked the adhesion and the establishment of biofilm. The exposure of both mycobacteria to MICs and sMICs lead to significant morphological changes: acquired a swollen form, ghost-like cell, disorganized cytoplasm detached from the cell wall. OD value of supernatant for both mycobacteria exposed to EOs have confirmed that there is a leakage of cellular material. Conclusion. The leakage of the cellular material is noticeably higher in sMIC, which is probably due to cell wall damage. sMIC of both EOs have an additive or synergistic effect, reducing MICs, limiting adhesion and preventing the formation of biofilms. |
| Databáze: | OpenAIRE |
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