Serotype-related differences in production and type of heat-labile hemolysin and heat-labile cytotoxin of Actinobacillus (Haemophilus) pleuropneumoniae
| Autor: | L.A.M.G. van Leengoed, E.M. Kamp |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Microbiology (medical)
Serotype Hot Temperature Swine Haemophilus Cross Reactions Hemolysin Proteins medicine.disease_cause Microbiology fluids and secretions Neutralization Tests medicine Animals Serotyping Pleuropneumonia Contagious Actinobacillus pleuropneumoniae Swine Diseases biology Cytotoxins Toxin Immune Sera Pasteurellaceae Hemolysin Actinobacillus bacterial infections and mycoses biology.organism_classification Virology digestive system diseases Specific Pathogen-Free Organisms bacteria Research Article |
| Zdroj: | Journal of Clinical Microbiology. 27:1187-1191 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.27.6.1187-1191.1989 |
| Popis: | Reference strains of serotypes 1 to 12 of Actinobacillus (Haemophilus) pleuropneumoniae were cultured in Eagle minimal essential medium with 10% Serum Plus. Culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. All strains except the reference strain of serotype 6 produced cytotoxin, whereas only serotypes 1, 5, 9, 10, and 11 produced hemolysin. Both cytotoxin and hemolysin appeared to be heat labile. Antisera were raised against cytotoxin- and hemolysin-containing culture supernatants of serotypes 1 to 11. Cross-neutralization studies revealed that the hemolysins were serologically homogeneous. In contrast, four serologically different cytotoxins were distinguished. One cytotoxin was produced by serotypes 1, 5, 9, and 11, and a second was produced by serotypes 2, 3, 4, and 8. A third cytotoxin was produced by serotypes 7 and 12; this cytotoxin was related to the cytotoxins of serotypes 1, 2, 4, 5, 9, and 11. A fourth cytotoxin, produced by serotype 10, was related to the cytotoxin of serotypes 1, 5, 9, and 11. Seventy field strains belonging to serotypes 2, 3, 7, 8, 9, and 11 were also tested for production of cytotoxin and hemolysin. All strains belonging to serotypes 9 and 11 produced hemolysin and cytotoxin, whereas all strains of serotypes 2, 3, 7, and 8 produced only cytotoxin. Hemolysins and cytotoxins of both the field strains and the corresponding serotype reference strains were comparably neutralized. These findings strongly suggest that the observed differences in production and type of hemolysin and cytotoxin were related to serotype and not to strain. |
| Databáze: | OpenAIRE |
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