Plasma Rich in Quercetin Metabolites Induces G2/M Arrest by Upregulating PPAR-γExpression in Human A549 Lung Cancer Cells
| Autor: | Shu-Ting Chan, Shu-Lan Yeh, Cheng-Hung Chuang, Chiao-Lin Yeh |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
G2 Phase
Male medicine.medical_specialty Lung Neoplasms Cell cycle checkpoint Cyclin B Administration Oral Pharmaceutical Science Analytical Chemistry Plasma chemistry.chemical_compound Cell Line Tumor Internal medicine CDC2 Protein Kinase Drug Discovery medicine Animals Humans Anilides heterocyclic compounds Phosphorylation Protein kinase B Cell Proliferation Cyclin Pharmacology A549 cell Cyclin-dependent kinase 1 biology Cell growth Organic Chemistry PTEN Phosphohydrolase Up-Regulation Oncogene Protein v-akt PPAR gamma Endocrinology Complementary and alternative medicine chemistry biology.protein Molecular Medicine Quercetin Gerbillinae Cell Division |
| Zdroj: | Planta Medica. 77:992-998 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0030-1250735 |
| Popis: | In this study, we incubated human A549 lung cancer cells with quercetin-metabolite-enriched plasma (QMP) obtained from Mongolian gerbils 2 h after quercetin feeding (100 mg/kg body wt/week). We investigated the effects of QMP on the growth of A549 cells and the possible mechanisms for these effects. We found that QMP but not control plasma (CP) reduced the cell growth in A549 cells. QMP led to cell cycle arrest at the G (2)/M phase by downregulating the expression of cdk1 and cyclin B. QMP but not CP or quercetin itself significantly increased PPAR- γ expression (p < 0.05), which was accompanied by an increase of phosphatase and tensin homologue deleted on the chromosome ten level and a decrease of phosphorylation of Akt. Furthermore, quercetin-3-glucuronide and quercetin-3'-sulfate also significantly increased PPAR- γ expression in A549 cells. GW9662, a PPAR- γ antagonist, significantly suppressed the effects of 10 % QMP on cell proliferation and on the expression of cyclin B and cdk1. Taken together, these data suggest that the activation of PPAR- γ plays an important role, at least in part, in the antiproliferative effects of quercetin metabolites. |
| Databáze: | OpenAIRE |
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