Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications
| Autor: | Seda Ozturan, Cristina Martínez-Ramos, Mikel Azkargorta, Eduardo Anitua, Ricardo Tejero, Isabel Goñi, Iñaki García-Arnáez, R. Izquierdo, Andreia Cerqueira, J. Suay, Felix Elortza, Mariló Gurruchaga, Francisco Romero-Gavilán |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
THP-1 Cells Biocompatible Materials plasma rich Biochemistry Monocytes bioinorganic chemistry CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA Desorption Materials Testing Titanium Blood clotting Bioinorganic chemistry proteomic analysis Cytokines biomaterials Surface Properties chemistry.chemical_element osteogenic differentiation Calcium blood clotting Osseointegration Inorganic Chemistry Adsorption proteomics dental implants growth-factors Cell Adhesion Humans coagulation Bone regeneration Original Paper calcium Osteoblasts Dental implants osseointegration Proteins implant surfaces chemistry Gene Expression Regulation inflammation Biophysics ions Protein adsorption |
| Zdroj: | Journal of Biological Inorganic Chemistry Addi. Archivo Digital para la Docencia y la Investigación instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia Repositori Universitat Jaume I Universitat Jaume I |
| ISSN: | 1432-1327 0949-8257 |
| Popis: | [EN] Calcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant-tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant-blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces. This work was supported by MINECO [MAT2017-86043-R; RTC-2017-6147-1], Generalitat Valenciana [GRISOLIAP/2018/091; APOSTD/2020/036, PROMETEO/2020/069], Universitat Jaume I under [ UJI-B2017-37], the University of the Basque Country under [GIU18/189] and Basque Government under [PRE_2017_2_0044]. The authors would like to thank Raquel Oliver, Jose Ortega and Iraide Escobes for their valuable technical assistance. |
| Databáze: | OpenAIRE |
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