Effect of spontaneous pathology and thrombin on leukocyte adhesion to rat aortic endothelium
| Autor: | María Gabaldón, Angel Zúñiga, Carmen Capdevila |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Pathology medicine.medical_specialty Endothelium Hirudin Biology Antibodies Viral Infections chemistry.chemical_compound Thrombin Cell Adhesion Leukocytes medicine Animals Mycoplasma Infections Platelet Aorta Platelet-activating factor Heparin Rats Inbred Strains Adhesion Antibodies Bacterial Rats medicine.anatomical_structure chemistry Coagulation Virus Diseases Mycoplasma pulmonis Endothelium Vascular Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Atherosclerosis. 93:217-228 |
| ISSN: | 0021-9150 |
| DOI: | 10.1016/0021-9150(92)90258-i |
| Popis: | Leukocyte adhesion and other injury parameters have been studied in the aortic endothelium of Sprague-Dawley rats in two situations: (1) spontaneous pathology in conventional rats with antibodies to Mycoplasma pulmonis and/or Kilham or Sendai viruses, and (2) intravascular coagulation by thrombin administration in SPF rats. Adhesion (esterase (+) leukocytes/mm 2 ) in SPF rats was 8 ± 5 ( n = 12). Adhesion in 38% of the conventional rats was 54 ± 27 ( n = 8), half of them being non-analyzed and the rest having antibodies to M. pulmonis and/or Kilham rat virus. In 19 rats with antibodies to M. pulmonis and/or Kilham or Sendai viruses, AgNO 3 and hematoxylin staining of the aortic endothelium showed an increase in leukocyte adhesion, and the presence of argyrophilic cells, stigmata and granularity — severe endothelial lesions being observed in some cases. Adhesion in rats after 0.25, 1, 3 and 6 h of thrombin administration (30 units/100 g) was not different from controls. Adhesion after 24 h was 108 ± 53 ( n = 10) and 60 ± 59 ( n = 10), and 22 ± 20 ( n = 10) in rats treated with thrombin plus heparin or hirudin, respectively. Thrombin produced endothelial lesions at all times studied, and these included membrane blebs, platelet and erythrocyte adhesion and alterations in the pattern of endothelial esterase activity. |
| Databáze: | OpenAIRE |
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