Surface hydrophobin prevents immune recognition of airborne fungal spores
| Autor: | Axel A. Brakhage, Jagadeesh Bayry, Jean-Paul Latgé, Cécile Clavaud, Sriramulu Elluru, Srini V. Kaveri, Sophie Paris, Silvia Bozza, Luigina Romani, Olaf Kniemeyer, Katia Perruccio, Vishukumar Aimanianda |
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| Přispěvatelé: | Aspergillus, Institut Pasteur [Paris], Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Université Sorbonne Paris Cité (USPC), Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Perugia (UNIPG), Leibniz Institute for Natural Product Research and Infection Biology (Hans Knoell Institute), Department of Clinical and Experimental Medicine, Linköping University (LIU), Université Grenoble Alpes (UGA), Friedrich-Schiller-Universität Jena, Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers ( CRC ), Université Paris Diderot - Paris 7 ( UPD7 ) -École pratique des hautes études ( EPHE ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), University of Perugia - Italy, University of Perugia, Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aimanianda, Vishukumar, Institut Pasteur [Paris] (IP), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Università degli Studi di Perugia = University of Perugia (UNIPG), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
MESH : Spores Fungal MESH: Macrophages Alveolar MESH : Hydrofluoric Acid [SDV]Life Sciences [q-bio] Galactosaminogalactan Air Microbiology Lymphocyte Activation [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Aspergillus fumigatus chemistry.chemical_compound MESH : Antigens Fungal Mice MESH: Air Microbiology MESH : Fungal Proteins MESH: Animals [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology MESH : Allergens [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Cells Cultured ComputingMilieux_MISCELLANEOUS 0303 health sciences Fungal protein Multidisciplinary biology MESH: Dendritic Cells MESH: Antigens Plant MESH : Adoptive Transfer MESH: CD4-Positive T-Lymphocytes Spores Fungal Adoptive Transfer [SDV] Life Sciences [q-bio] MESH : Macrophages Alveolar MESH : CD4-Positive T-Lymphocytes MESH: Fungal Proteins MESH: Aspergillus fumigatus Cladosporium MESH: Cells Cultured MESH: Allergens Antigens Fungal Hydrophobin MESH : Air Microbiology MESH : Mice Inbred C57BL MESH : Cathepsins Hydrofluoric Acid Microbiology MESH: Hydrofluoric Acid Fungal Proteins 03 medical and health sciences Immune system MESH: Mice Inbred C57BL MESH: Spores Fungal MESH : Mice MESH : Cells Cultured Macrophages Alveolar Animals Humans MESH: Lymphocyte Activation [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity MESH: Mice MESH : Lymphocyte Activation 030304 developmental biology MESH: Antigens Fungal [ SDV.IMM.II ] Life Sciences [q-bio]/Immunology/Innate immunity Innate immune system MESH: Humans MESH: Immune System [ SDV ] Life Sciences [q-bio] 030306 microbiology fungi MESH : Humans Dendritic cell Dendritic Cells Allergens Antigens Plant biology.organism_classification [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Cathepsins Mice Inbred C57BL MESH: Adoptive Transfer MESH : Antigens Plant chemistry MESH : Dendritic Cells MESH : Aspergillus fumigatus Immune System MESH: Cathepsins MESH : Immune System MESH : Animals |
| Zdroj: | Nature Nature, Nature Publishing Group, 2009, 460 (7259), pp.1117-1121. ⟨10.1038/nature08264⟩ Nature, Nature Publishing Group, 2009, 460 (7259), pp.1117-1121. 〈10.1038/nature08264〉 Nature, 2009, 460 (7259), pp.1117-1121. ⟨10.1038/nature08264⟩ |
| ISSN: | 0028-0836 1476-4679 1476-4687 |
| DOI: | 10.1038/nature08264⟩ |
| Popis: | International audience; The air we breathe is filled with thousands of fungal spores (conidia) per cubic metre, which in certain composting environments can easily exceed 10(9) per cubic metre. They originate from more than a hundred fungal species belonging mainly to the genera Cladosporium, Penicillium, Alternaria and Aspergillus. Although these conidia contain many antigens and allergens, it is not known why airborne fungal microflora do not activate the host innate immune cells continuously and do not induce detrimental inflammatory responses following their inhalation. Here we show that the surface layer on the dormant conidia masks their recognition by the immune system and hence prevents immune response. To explore this, we used several fungal members of the airborne microflora, including the human opportunistic fungal pathogen Aspergillus fumigatus, in in vitro assays with dendritic cells and alveolar macrophages and in in vivo murine experiments. In A. fumigatus, this surface 'rodlet layer' is composed of hydrophobic RodA protein covalently bound to the conidial cell wall through glycosylphosphatidylinositol-remnants. RodA extracted from conidia of A. fumigatus was immunologically inert and did not induce dendritic cell or alveolar macrophage maturation and activation, and failed to activate helper T-cell immune responses in vivo. The removal of this surface 'rodlet/hydrophobin layer' either chemically (using hydrofluoric acid), genetically (DeltarodA mutant) or biologically (germination) resulted in conidial morphotypes inducing immune activation. All these observations show that the hydrophobic rodlet layer on the conidial cell surface immunologically silences airborne moulds. |
| Databáze: | OpenAIRE |
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