Nanotechnological Applications Hold a Pivotal Position in Boosting Stem Cells Osteogenic Activity: In Vitro and In Vivo Studies
| Autor: | Riham M Aly, Nadia S. Mahmoud, Naglaa A. Ali, Hadeer A. Aglan, Hanaa H. Ahmed, Ahmed A. Abd-Rabou |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Bone Marrow Cells Bioengineering Applied Microbiology and Biotechnology Biochemistry Bone remodeling Microscopy Electron Transmission X-Ray Diffraction Osteogenesis In vivo Spectroscopy Fourier Transform Infrared medicine Animals Nanotechnology MTT assay Rats Wistar Molecular Biology Chemistry Stem Cells Mesenchymal stem cell Cell Differentiation Osteoblast General Medicine Coculture Techniques In vitro Rats Cell biology medicine.anatomical_structure Microscopy Electron Scanning Ovariectomized rat Female Stem cell Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 190:551-573 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | This approach was constructed to appraise the therapeutic effectiveness of a single i.v. dose of osteoblasts generated from co-culturing BM-MSCs with nano-HA, Pt-NPs, or Pt-HA-nanocomposite in osteoporotic rats. MSCs were grown, propagated in culture, and characterized. The effect of the suggested nanoplatforms on the survival, osteogenic differentiation, and mineralization of BM-MSCs was assessed by MTT assay, real-time PCR analysis, and Alizarin red S staining, respectively. Thereafter, the generated osteoblasts were employed for the treatment of ovariectomized rats. Our results revealed that the selected nanoplatforms upregulate the expression of osteogenic differentiation related genes (Runx-2 and BMP-2) significantly and enhance calcium deposition in BM-MSCs after 7 and 21 days, respectively, whereas the in vivo study validated that the infusion of the generated osteoblasts considerably downturn serum BALP, BSP, and SOST levels; upswing OSX level; and regain femur bone mineralization and histoarchitecture. Conclusively, the outcomes of this work provide scientific evidence that transplanting osteoblasts derived from differentiation of BM-MSCs in the presence of nanoplatforms in ovariectomized rats restores bone remodeling balance which constitutes a new hope for the treatment of osteoporosis. |
| Databáze: | OpenAIRE |
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