Anti-CD3-Activated T Cells from Chronic Nonviremic HBV Carriers Are Hyperreactive to Monocytic Accessory Signals
| Autor: | Firelei L. Sirit, Diliam J. Caldera, Nicolas E. Bianco, Mercedes Zabaleta, Carmen J. Colmenares, Felix Toro, Irma Machado, Miren L. Baroja |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Interleukin 2 Hepatitis B virus CD3 Complex T-Lymphocytes Receptor expression T cell Immunology Dose-Response Relationship Immunologic chemical and pharmacologic phenomena Biology Lymphocyte Activation Peripheral blood mononuclear cell Monocytes Pathology and Forensic Medicine medicine Humans Immunology and Allergy Antigen-presenting cell Monocyte Autologous Monocytes Receptors Interleukin-2 T lymphocyte Middle Aged Hepatitis B Molecular biology medicine.anatomical_structure Carrier State DNA Viral Interleukin-2 Female medicine.drug |
| Zdroj: | Clinical Immunology and Immunopathology. 69:180-188 |
| ISSN: | 0090-1229 |
| Popis: | We analyzed T cell responses through the CD3 activation pathway in a group of chronic HBV carriers. PBMC stimulated with the mAb OKT3 showed higher proliferative response in HBV-DNA(-) carriers compared to HBV-DNA(+) carriers and to controls. In contrast, no differences in proliferative responses were observed between HBV-DNA(-) carriers and controls in cell cultures stimulated with immobilized 64.1 mAb (SPB-64.1) which induces proliferation in the absence of monocytes. We further examined T cell responses in the presence of monocytes and their soluble factors to immobilized OKT3 mAb (SPB-OKT3). Purified T cells did not proliferate to SPB-OKT3. When autologous monocytes were added, higher proliferative response, IL-2 production, and IL-2 receptor expression were observed in HBV-DNA(-) carriers than in controls. An enhanced cell proliferation was also obtained when monocyte supernatants were added to T cells cultured with SPB-OKT3. Moreover, when IL-6 alone or combined with IL-1 was added to SPB-OKT3-stimulated T cell cultures, a significantly higher increase in T cell proliferation was detected in HBV-DNA(-) carriers. Our results thus show a T cell hyperreactivity to accessory signals from monocytes (mainly IL-6) in HBV-DNA(-) carriers, that is probably related to an ongoing viral clearance. |
| Databáze: | OpenAIRE |
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