Effect of antibiotic gut microbiota disruption on LPS-induced acute lung inflammation
| Autor: | Tom van der Poll, Nora S. Wolff, Alex F. de Vos, Floor Hugenholtz, Jacqueline M. Lankelma, W. Joost Wiersinga, Max C. Jacobs |
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| Přispěvatelé: | Graduate School, AII - Infectious diseases, Center of Experimental and Molecular Medicine, Infectious diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Lipopolysaccharide Physiology Neutrophils Gut flora Toxicology Pathology and Laboratory Medicine chemistry.chemical_compound White Blood Cells Mice Medical Conditions Antibiotics Animal Cells Immune Physiology Natural Resources Medicine and Health Sciences Toxins Immune Response Lung Innate Immune System Multidisciplinary medicine.diagnostic_test biology Antimicrobials Interleukin Drugs respiratory system Anti-Bacterial Agents medicine.anatomical_structure Water Resources Cytokines Medicine Tumor necrosis factor alpha Female medicine.symptom Cellular Types Bronchoalveolar Lavage Fluid Research Article Inflammatory Diseases Immune Cells Science 030106 microbiology Immunology Toxic Agents Bacterial Toxins Acute Lung Injury Inflammation Microbiology 03 medical and health sciences Signs and Symptoms Microbial Control medicine Animals Pharmacology Blood Cells Bacteria business.industry Interleukin-6 Gut Bacteria Ecology and Environmental Sciences Organisms Biology and Life Sciences Cell Biology Molecular Development medicine.disease biology.organism_classification respiratory tract diseases Gastrointestinal Microbiome Endotoxins Mice Inbred C57BL Pneumonia 030104 developmental biology Bronchoalveolar lavage chemistry Immune System Clinical Medicine business Developmental Biology |
| Zdroj: | PLoS ONE, Vol 15, Iss 11, p e0241748 (2020) PLoS ONE PLoS ONE, 15(11 November):e0241748. Public Library of Science |
| ISSN: | 1932-6203 |
| Popis: | BackgroundAn increasing body of evidence is indicating that the gut microbiota modulates pulmonary inflammatory responses. This so-called gut-lung axis might be of importance in a whole spectrum of inflammatory pulmonary diseases such as acute respiratory distress syndrome, chronic obstructive pulmonary disease and pneumonia. Here, we investigate the effect of antibiotic disruption of gut microbiota on immune responses in the lung after a intranasal challenge with lipopolysaccharide (LPS).Methods/resultsC57Bl/6 mice were treated for two weeks with broad-spectrum antibiotics supplemented to their drinking water. Afterwards, mice and untreated control mice were inoculated intranasally with LPS. Mice were sacrificed 2 and 6 hours post-challenge, after which bronchoalveolar lavage fluid (BALF) and lung tissues were taken. Gut microbiota analysis showed that antibiotic-treated mice had a pronounced reduction in numbers and diversity of bacteria. A modest, but time consistent, significant increase of interleukin (IL)-6 release was seen in BALF of antibiotic treated mice. Release of tumor necrosis factor alpha (TNFα), however, was not statistically different between groups.ConclusionAntibiotic induced microbiota disruption is associated with alterations in host responses during LPS-induced lung inflammation. Further studies are required to determine the clinical relevance of the gut-lung axis in pulmonary infection and inflammation. |
| Databáze: | OpenAIRE |
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