A novel gene is transcribed in the chicken α-globin gene domain in the direction opposite to the globin genes
| Autor: | Olga V. Iarovaia, Klaus Scherrer, Nikolajs Sjakste, V. Le Gac, Tatjana Sjakste, Sergey V. Razin, Z. Zhao, G. Linares-Cruz |
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| Předmět: |
Cytoplasm
Nuclear gene Transcription Genetic Molecular Sequence Data Pair-rule gene Biology Cell Line Gene product Exon Mice Species Specificity Sequence Homology Nucleic Acid Gene cluster Genetics Animals Humans RNA Antisense RNA Messenger Molecular Biology Gene Regulator gene Cell Nucleus Base Sequence Gene targeting Chromosome Mapping Exons RNA Probes Molecular biology Globins Multigene Family Chickens |
| Zdroj: | Scopus-Elsevier |
| Popis: | A novel gene transcribed in the direction opposite to that of the globin genes was found in the chicken alpha-globin gene domain. Northern hybridisation with single-stranded riboprobes revealed that a 4.5-kb poly(A)+ RNA is transcribed in antisense polarity with respect to the globin genes. The transcription unit encoding this RNA seems to overlap the entire cluster of alpha-globin genes and extends at least 15 kb upstream from pi, the first of the alpha-globin genes. This new transcript shows partial sequence homology with that encoded by the human "-14" gene. An oligonucleotide based on part of a restriction fragment of chicken DNA that is 80% homologous to exon 4 of the human "-14" gene hybridises with a 4.5-kb RNA molecule. In situ hybridisation of globin-antisense probes, that detect polyribosomal mRNAs of 1.7 and 2.5 kb on Northern blots, shows these "antisense" transcripts to be present in the cytoplasm. The 4.5-kb RNA is absent in polyribosomal poly(A)+ RNA and may, hence, represent a nuclear pre-mRNA transcribed from the chicken gene that is homologous to the human "-14" gene. The expression of this gene is not specific to erythroid cells; analogous transcripts were also detected in poly(A)+ RNA extracted from a chicken lymphoblastoma cell line (HP50). Taken together, these data allow us to postulate the existence in the chicken genome of a novel gene, for which we suggest the name "ggPRX" in analogy to the murine mProx1, a gene identified in the upstream region of the alpha-globin gene domain in mice. |
| Databáze: | OpenAIRE |
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