An improved technique for Ia-determination in guinea pigs
| Autor: | J Møller, J T Clausen, J V Spärck |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cytotoxicity
Immunologic Isoantigens Rosette Formation T-Lymphocytes Lymphocyte Guinea Pigs Immunology Biology Major histocompatibility complex Biochemistry Guinea pig Epitopes Species Specificity Genetics medicine Animals Immunology and Allergy Typing Cytotoxicity B-Lymphocytes medicine.diagnostic_test Histocompatibility Antigens Class II General Medicine Molecular biology Peripheral blood medicine.anatomical_structure biology.protein Lymph Tissue typing |
| Zdroj: | Tissue Antigens. 23:41-46 |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1984.tb00006.x |
| Popis: | The serological typing of determinants within the guinea pig histocompatibility complex (GPLA-complex) has, until now, largely been carried out by the 51Cr-release technique. As target cells, a mixture of B and T cells from peripheral blood and lymph nodes were used. However, for the detection of Ia-determinants, which predominantly are expressed on the surface of B cells, the use of lymphocyte suspensions with a relatively low content of B cells can cause some evaluation problems. We have developed a simple method for obtaining a relatively pure suspension of B cells, making use of the ability of guinea pig T cells to form rosettes with rabbit red blood cells and the possibility of removing these by sedimentation on lymphoflot. By employing these purified B cells, an improved serological detection of Ia-determinants of the guinea pig histocompatibility complex is made possible. |
| Databáze: | OpenAIRE |
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