Phosphatidylethanolamine and Cardiolipin Differentially Affect the Stability of Mitochondrial Respiratory Chain Supercomplexes
| Autor: | Thomas Kleinschroth, Nikolaus Pfanner, Günther Daum, Susanne E. Horvath, Lena Böttinger, Thomas Becker, Carola Hunte |
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| Rok vydání: | 2012 |
| Předmět: |
Psd1
CL cardiolipin Psd2 Cardiolipins Respiratory chain Saccharomyces cerevisiae Biology PE phosphatidylethanolamine Electron Transport Electron Transport Complex IV chemistry.chemical_compound Structural Biology Crd1 cardiolipin synthase Cardiolipin Inner membrane Cytochrome c oxidase AAC ADP/ATP carrier TIM22 carrier translocase of the inner mitochondrial membrane Inner mitochondrial membrane Molecular Biology Psd phosphatidylserine decarboxylase Communication TIM23 presequence translocase of the inner mitochondrial membrane Phosphatidylethanolamines F1β β subunit of F1Fo-ATP synthase Mitochondria Cell biology Protein Transport Mitochondrial respiratory chain chemistry Coenzyme Q – cytochrome c reductase Translocase of the inner membrane biology.protein membrane potential protein import |
| Zdroj: | Journal of Molecular Biology |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/j.jmb.2012.09.001 |
| Popis: | The mitochondrial inner membrane contains two non-bilayer‐forming phospholipids, phosphatidylethanolamine (PE) and cardiolipin (CL). Lack of CL leads to destabilization of respiratory chain supercomplexes, a reduced activity of cytochrome c oxidase, and a reduced inner membrane potential Δψ. Although PE is more abundant than CL in the mitochondrial inner membrane, its role in biogenesis and assembly of inner membrane complexes is unknown. We report that similar to the lack of CL, PE depletion resulted in a decrease of Δψ and thus in an impaired import of preproteins into and across the inner membrane. The respiratory capacity and in particular the activity of cytochrome c oxidase were impaired in PE-depleted mitochondria, leading to the decrease of Δψ. In contrast to depletion of CL, depletion of PE did not destabilize respiratory chain supercomplexes but favored the formation of larger supercomplexes (megacomplexes) between the cytochrome bc1 complex and the cytochrome c oxidase. We conclude that both PE and CL are required for a full activity of the mitochondrial respiratory chain and the efficient generation of the inner membrane potential. The mechanisms, however, are different since these non-bilayer‐forming phospholipids exert opposite effects on the stability of respiratory chain supercomplexes. Graphical Abstract Highlights ► PE required for full respiratory capacity of mitochondria. ► Membrane potential and protein import are impaired in PE-depleted mitochondria. ► PE depletion stabilizes respiratory chain supercomplexes (megacomplexes). ► PE and CL exert antagonistic effects on respiratory chain supercomplexes. |
| Databáze: | OpenAIRE |
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