Immunogenicity of a Locally Produced Newcastle Disease I-2 Thermostable Vaccine in Chickens in Uganda
| Autor: | J Illango, W. Olaho-Mukani, P. P. Abila, G Mukiibi-Muka, A Etoori |
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| Rok vydání: | 2005 |
| Předmět: |
Veterinary medicine
Newcastle Disease Drinking Newcastle disease virus Enzyme-Linked Immunosorbent Assay Antibodies Viral Newcastle disease Vaccine administration Adjuvants Immunologic Food Animals medicine Animals Uganda Poultry Diseases biology Drug Administration Routes Immunogenicity Vaccination Viral Vaccines Levamisole biology.organism_classification Serum samples Virology Specific Pathogen-Free Organisms biology.protein Animal Science and Zoology Ophthalmic Solutions Antibody Chickens La Sota vaccine medicine.drug |
| Zdroj: | Tropical Animal Health and Production. 37:25-31 |
| ISSN: | 0049-4747 |
| DOI: | 10.1023/b:trop.0000047933.52512.c5 |
| Popis: | A locally-produced Newcastle disease (ND) I-2 thermostable vaccine of embryo-infective dose (EID50) 10(8.5) per ml was administered to 100 laboratory chickens in four test groups, each of 25 birds. It was given by the eye-drop method, in drinking water, in drinking water freshly medicated with levamisole, or using millet grains as a vaccine carrier. A fifth control group consisting of 25 birds received the heat-sensitive La Sota vaccine (EID50 10(9) per ml) by the eye-drop method. The immunological responses were monitored by the enzyme-linked immunosorbent assay (ELISA) ND antibody technique using serum samples collected from 18 birds in each group at 3-week intervals for 3 months. The overall mean ND antibody log(10) titres and percentage positivities were 3.1, 88%; 2.9, 70%; 3.0, 83%; 3.2, 87% and 3.3, 87%, respectively. The use of water alone or medicated with levamisole for vaccine administration produced significantly lower ND antibody titres only in the first 3 weeks. The immunogenicity shown by the I-2 vaccine as a potential vaccine is discussed in relation to free-range poultry management conditions in Uganda. |
| Databáze: | OpenAIRE |
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