Pairing of homologous chromosomes inC. elegansmeiosis requires DEB-1 - an orthologue of mammalian vinculin
| Autor: | Lenka Hůlková, Petr Flachs, Pavel Hozák, Jana Fukalová, Jana Rohožková |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:QH426-470
LINC complex Chromosomes 03 medical and health sciences Meiotic Prophase I Meiosis Homologous chromosome Animals lcsh:QH573-671 Caenorhabditis elegans 030304 developmental biology 0303 health sciences deb-1 biology vinculin lcsh:Cytology c. elegans 030302 biochemistry & molecular biology Chromosome Cell Biology Vinculin linc complex Cell biology lcsh:Genetics Synaptonemal complex chromosome pairing Pairing prophase i biology.protein Research Paper |
| Zdroj: | Nucleus, Vol 10, Iss 1, Pp 93-115 (2019) Nucleus |
| ISSN: | 1949-1042 1949-1034 |
| Popis: | During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this ‘chromosomal dance’ still remainunclear. We identified DEB-1 – an orthologue of mammalian vinculin – as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINC complex. In early meiotic nuclei of C. elegans, DEB-1 is localized to the nuclear periphery and alongside the synaptonemal complex of paired homologues. Upon DEB-1 depletion, chromosomes attached to SUN-1 foci remain highly motile until late pachytene. Although the initiation of homologue pairing started normally, irregularities in the formation of the synaptonemal complex occur, and these results in meiotic defects such as increased number of univalents at diakinesis and high embryonic lethality. Our data identify DEB-1 as a new player regulating chromosome dynamics and pairing during meiotic prophase I. |
| Databáze: | OpenAIRE |
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