A Mouse Model of Small-Vessel Disease that Produces Brain-Wide-Identified Microocclusions and Regionally Selective Neuronal Injury
| Autor: | Gergely Silasi, Jamie D. Boyd, Timothy H. Murphy, Jennifer She, Songchao Xue |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Genetically modified mouse Pathology medicine.medical_specialty Transgene Thalamus Hippocampus Arterial Occlusive Diseases Mice Transgenic Striatum Biology Brief Communication White matter Mice medicine Animals Humans Neocortex Histology White Matter Axons Microspheres Arterioles Cerebrovascular Disorders medicine.anatomical_structure nervous system Neurology Female Neurology (clinical) Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 35:734-738 |
| ISSN: | 1559-7016 0271-678X |
| DOI: | 10.1038/jcbfm.2015.8 |
| Popis: | We developed a mouse model of small-vessel disease where occlusions are produced through endovascular injection of fluorescent microspheres that target ~12 μm diameter penetrating arterioles and can be localized in histology. Using Thy1-GFP transgenic mice, we visualized the impact of microocclusions on neuronal structure. Microocclusions in the hippocampus produce cell loss or neuronal atrophy (~7% of lodged microspheres led to microinfarcts), while axons within white matter tracts, as well as the striatum and thalamus became blebbed or disrupted. Although the neocortex contained more occlusions than other structures, labeled layer 5 neurons were relatively resistant to structural damage, with |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|