Contact inhibition of locomotion and mechanical cross-talk between cell-cell and cell-substrate adhesion determines the pattern of junctional tension in epithelial cell aggregates
| Autor: | Hender Lopez, Benjamin J. Caldwell, Guillermo A. Gomez, Vladimir Lobaskin, Anthony P. Roberts, Chloe X. Yap, Elliott Moussa, Adrian Noppe, Alpha S. Yap, Zoltan Neufeld, Rashmi Priya, Luke Coburn |
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| Přispěvatelé: | Coburn, Luke, Lopez, Hender, Caldwell, Benjamin J, Moussa, Elliott, Yap, Chloe, Priya, Rashmi, Noppe, Adrian, Roberts, Anthony P, Lobaskin, Vladimir, Yap, Alpha S, Neufeld, Zoltan, Gomez, Guillermo A |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell FOS: Physical sciences Cell Communication Biology Immunofluorescence Models Biological Cell junction Epithelium 03 medical and health sciences Cell Movement physics - biological physics Cell Behavior (q-bio.CB) Cell Adhesion medicine Animals Humans Computer Simulation quantitative biology - cell behavior Physics - Biological Physics Cell Interactions Cell-substrate adhesion Tissues and Organs (q-bio.TO) Molecular Biology medicine.diagnostic_test Contact Inhibition Contact inhibition Epithelial Cells Quantitative Biology - Tissues and Organs Receptor Cross-Talk Articles Cell Biology quantitative biology - tissues and organs Cell biology Intercellular Junctions 030104 developmental biology medicine.anatomical_structure Homogeneous Biological Physics (physics.bio-ph) FOS: Biological sciences Quantitative Biology - Cell Behavior Cell Surface Extensions Locomotion |
| Zdroj: | ResearcherID Molecular Biology of the Cell |
| DOI: | 10.48550/arxiv.1604.03733 |
| Popis: | We generated a computational approach to analyze the biomechanics of epithelial cell aggregates, either island or stripes or entire monolayers, that combines both vertex and contact-inhibition-of-locomotion models to include both cell-cell and cell-substrate adhesion. Examination of the distribution of cell protrusions (adhesion to the substrate) in the model predicted high order profiles of cell organization that agree with those previously seen experimentally. Cells acquired an asymmetric distribution of basal protrusions, traction forces and apical aspect ratios that decreased when moving from the edge to the island center. Our in silico analysis also showed that tension on cell-cell junctions and apical stress is not homogeneous across the island. Instead, these parameters are higher at the island center and scales up with island size, which we confirmed experimentally using laser ablation assays and immunofluorescence. Without formally being a 3-dimensional model, our approach has the minimal elements necessary to reproduce the distribution of cellular forces and mechanical crosstalk as well as distribution of principal stress in cells within epithelial cell aggregates. By making experimental testable predictions, our approach would benefit the mechanical analysis of epithelial tissues, especially when local changes in cell-cell and/or cell-substrate adhesion drive collective cell behavior. Comment: 39 pages, 8 Figures. Supplementary Information is included |
| Databáze: | OpenAIRE |
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