Novel Helicobacter pylori Sequencing Test Identifies High Rate of Clarithromycin Resistance
| Autor: | Jason Y. Park, Ashish S. Patel, Christopher D. Doern, N. Kristine Leos, Midori Mitui |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Male Adolescent DNA Mutational Analysis Population Drug resistance Polymerase Chain Reaction Sensitivity and Specificity Helicobacter Infections Microbiology law.invention Young Adult Antibiotic resistance 23S ribosomal RNA law Clarithromycin Drug Resistance Bacterial medicine Humans Treatment Failure Child education Polymerase chain reaction DNA Primers education.field_of_study Helicobacter pylori biology Hybridization probe Gastroenterology bacterial infections and mycoses biology.organism_classification Virology Anti-Bacterial Agents RNA Ribosomal 23S Child Preschool Retreatment Pediatrics Perinatology and Child Health Female medicine.drug |
| Zdroj: | Journal of Pediatric Gastroenterology & Nutrition. 59:6-9 |
| ISSN: | 0277-2116 |
| Popis: | Objectives: Eradication therapy selection for Helicobacter pylori gastritis requires knowledge of the local resistance rate to clarithromycin. There is minimal population-based or regional data in the United States on pediatric clarithromycin resistance. Although commercial methods such as fluorescence in situ hybridization and DNA probe assays are available in Europe for the evaluation of H pylori 23S rRNA mutations associated with resistance, clinical testing for 23S rRNA in the United States is not widely available.Thisstudyexaminedasinglepediatric institution’sclarithromycin resistance rate by a DNA polymerase chain reaction/sequencing assay applied to archived gastric biopsy specimens. Methods: From the period 2010 to 2012, 38 H pylori‐infected gastric biopsies were examined from archived formalin-fixed paraffin-embedded (FFPE) material. The 23S rRNA gene of H pylori was polymerase chain reaction amplified and sequenced for the identification of point mutations that are associated with clarithromycin therapeutic resistance. Results: By 23S rRNA gene sequencing, 50% (n ¼19) of the specimens contained H pylori with mutations significant for clarithromycin resistance. Conclusions:This studyis consistentwith other pediatricreports suggesting significant H pylori clarithromycin resistance in the United States. Furthermore, the method used in this study can be used by hospitalbased clinical laboratories to assess local clarithromycin resistance from archived biopsy material. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text