Simultaneous radioenzymatic determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma of patients with hepatic encephalopathy
| Autor: | Livio Capocaccia, F. Rossi Fanelli, A. Bozzi, Oliviero Riggio, S. Gentile, P. Cardelli-Cangiano, Roberto Strom, Carlo Cangiano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1981 |
| Předmět: |
S-Adenosylmethionine
hepatic encephalopathy neurotransmitter agents octopamine phenylethylamine tyramine Ethanol Chromatography Clinical Biochemistry Extraction (chemistry) General Medicine Tyramine medicine.disease Toluene Solvent Phenylethanolamine chemistry.chemical_compound chemistry Ethanolamines Phenethylamines medicine Humans Octopamine (neurotransmitter) Hepatic encephalopathy |
| Popis: | A sensitive radioenzymatic assay for the simultaneous determination of phenylethylamine, phenylethanolamine, tyramine and octopamine in plasma samples is reported. After extraction with ethanol, the amines are subjected to enzymatic N-methylation with labelled S-adenosylmethionine, preceded in the case of phenylethylamine and of tyramine, by an enzyme-catalyzed beta-hydroxylation step. The procedure is completed by extraction with toluene containing different amounts of isoamylalcohol, followed by controlled evaporation of the solvent. A generalized and highly significant increase in the concentration of all the four amines was found in plasma samples from patients suffering from hepatic encephalopathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|