A Competitive Pull-Down Assay Using G-quadruplex DNA Linked Magnetic Nanoparticles To Determine Specificity of G-quadruplex Ligands
| Autor: | Velayutham Ravichandiran, Thumpati Prasanth, Ritapa Chaudhuri, Puja Saha, Deepanjan Panda, Jyotirmayee Dash |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Azides
Immunoprecipitation Ligands 010402 general chemistry G-quadruplex 01 natural sciences Catalysis Analytical Chemistry Proto-Oncogene Proteins c-myc chemistry.chemical_compound Cell Line Tumor Screening method Humans heterocyclic compounds Magnetite Nanoparticles Promoter Regions Genetic Cycloaddition Reaction 010401 analytical chemistry Triazoles Small molecule Combinatorial chemistry Ferrosoferric Oxide Cycloaddition 0104 chemical sciences G-Quadruplexes Proto-Oncogene Proteins c-bcl-2 chemistry Alkynes Cancer cell Magnetic nanoparticles Copper DNA |
| Zdroj: | Analytical Chemistry. 91:7705-7711 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Herein, we develop a competitive screening method in which G-quadruplex DNA linked magnetic nanoparticles pull down selective ligands for a particular quadruplex topology from a series of small molecules. The screening strategy is first optimized with known G-quadruplex ligands and then used with a new series of G-quadruplex interactive bis-triazolyl ligands that are synthesized by Cu(I)-catalyzed azide-alkyne cycloaddition. The assay enables the identification of c-MYC and BCL2 G-quadruplex selective bis-triazole ligands that specifically target promoter G-quadruplexes in cancer cells. |
| Databáze: | OpenAIRE |
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