Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov
| Autor: | Ayumi Akiyoshi, Hisayoshi Nozaki, Hiroshi Kawai, Hiroshi Mori, Masahiro Suzuki, Akira Kurihara, Hidetoshi Sakayama, Kazuhiro Kogame, Taiju Kitayama, Takahiro Segawa, Tsuyoshi Abe, Ryo Ootsuki |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Medicine Biochemistry 01 natural sciences Japonica Database and Informatics Methods DNA Algal Japan Genus Liagora japonica lcsh:Science Phylogeny Multidisciplinary Phylogenetic tree High-Throughput Nucleotide Sequencing food and beverages Phylogenetic Analysis Genomics Genomic Databases humanities Nucleic acids Ribosomal RNA Otohimella Sequence Analysis Research Article Cell biology Multiple Alignment Calculation Cellular structures and organelles Sequence Databases Red algae Biology Research and Analysis Methods DNA Ribosomal DNA sequencing Specimen Handling 03 medical and health sciences Terminology as Topic Computational Techniques Botany parasitic diseases Genetics Molecular Biology Techniques Sequencing Techniques Non-coding RNA Molecular Biology DNA sequence analysis Molecular Biology Assays and Analysis Techniques Sequence Assembly Tools Pacific Ocean 010604 marine biology & hydrobiology fungi lcsh:R Biology and Life Sciences Computational Biology Genes rRNA Genome Analysis biology.organism_classification Split-Decomposition Method Biological Databases 030104 developmental biology Rhodophyta RNA Type locality lcsh:Q Ribosomes Sequence Alignment |
| Zdroj: | PLoS ONE, Vol 11, Iss 7, p e0158944 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Liagora japonica is a red algal species distributed in temperate regions of Japan. This species has not been collected from its type locality on the Pacific coast of Japan since 1927 and seems to have become extinct in this area. For molecular characterization of L. japonica, we extracted DNA from the topotype material of L. japonica collected in 1927, analyzed seven genes using Illumina next-generation sequencing, and compared these data with sequences from modern samples of similar red algae collected from the Japan Sea coast of Japan. Both morphological and molecular data from modern samples and historical specimens (including the lectotype and topotype) suggest that the specimens from the Pacific and Japan Sea coasts of Japan should be treated as a single species, and that L. japonica is phylogenetically separated from the genus Liagora. Based on the phylogenetic results and examination of reproductive structures, we propose Otohimella japonica gen. et comb. nov., characterized morphologically by diffuse carposporophytes, undivided carposporangia, and involucral filaments initiated only from the cortical cell on the supporting cell. |
| Databáze: | OpenAIRE |
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