Blood meal-induced inhibition of vector-borne disease by transgenic microbiota
| Autor: | David J. Lampe, Jackie L. Shane, Christina L. Grogan, Caroline Cwalina |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Plasmodium berghei Science 030106 microbiology Population General Physics and Astronomy Mosquito Vectors Plasmodium Article General Biochemistry Genetics and Molecular Biology Microbiology Animals Genetically Modified 03 medical and health sciences Anopheles Antibiosis parasitic diseases Animals lcsh:Science Symbiosis education Anopheles stephensi Pathogen Disease Resistance education.field_of_study Multidisciplinary biology Effector Microbiota fungi General Chemistry biology.organism_classification Blood meal Malaria 3. Good health Vector (epidemiology) Acetobacteraceae lcsh:Q Digestive System |
| Zdroj: | Nature Communications Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-06580-9 |
| Popis: | Vector-borne diseases are a substantial portion of the global disease burden; one of the deadliest of these is malaria. Vector control strategies have been hindered by mosquito and pathogen resistances, and population alteration approaches using transgenic mosquitos still have many hurdles to overcome before they can be implemented in the field. Here we report a paratransgenic control strategy in which the microbiota of Anopheles stephensi was engineered to produce an antiplasmodial effector causing the mosquito to become refractory to Plasmodium berghei. The midgut symbiont Asaia was used to conditionally express the antiplasmodial protein scorpine only when a blood meal was present. These blood meal inducible Asaia strains significantly inhibit pathogen infection, and display improved fitness compared to strains that constitutively express the antiplasmodial effector. This strategy may allow the antiplasmodial bacterial strains to survive and be transmitted through mosquito populations, creating an easily implemented and enduring vector control strategy. Vector alteration strategies are emerging as attractive tools for malaria transmission control. Here, Shane et al. engineer a bacterial strain, isolated from mosquitoes, to produce an antiplasmodial protein in the presence of blood meal, causing the mosquitoes to become refractory to Plasmodium infection. |
| Databáze: | OpenAIRE |
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