Visualization of ultraviolet absorption distribution beyond the diffraction limit of light by electron-beam excitation-assisted optical microscope
| Autor: | Yoshimasa Kawata, Susumu Terakawa, Wataru Inami, Masahiro Fukuta |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Diffraction
Histology Materials science genetic structures Physics::Optics 02 engineering and technology medicine.disease_cause Pathology and Forensic Medicine law.invention Crystal 03 medical and health sciences Optics Optical microscope law Condensed Matter::Superconductivity medicine Absorption (electromagnetic radiation) 030304 developmental biology 0303 health sciences business.industry 021001 nanoscience & nanotechnology Fluorescence Wavelength Near-field scanning optical microscope sense organs 0210 nano-technology business Ultraviolet |
| Zdroj: | Journal of microscopyReferences. 276(1) |
| ISSN: | 1365-2818 |
| Popis: | We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 has absorption at ultraviolet wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film of an electron-beam excitation-assisted (EXA) optical microscope. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal. The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA optical microscope is useful for analysis of growth of a crystal, bioimaging and so on. |
| Databáze: | OpenAIRE |
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