Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter
| Autor: | Howard C. Hang, Markus Grammel, Emma Taylor-Salmon, Paul D. Dossa |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Salmonella typhimurium
Cell type Proteome Cell Phenylalanine Methionine-tRNA Ligase Catalysis chemistry.chemical_compound Materials Chemistry medicine Humans Receptors Amino Acid Pathogen chemistry.chemical_classification Alanine Chemistry Aminoacyl tRNA synthetase Metals and Alloys General Chemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Amino acid medicine.anatomical_structure Biochemistry Microscopy Fluorescence Isotope Labeling Ceramics and Composites Intracellular HeLa Cells |
| Zdroj: | Chemical communications (Cambridge, England). 48(10) |
| ISSN: | 1364-548X |
| Popis: | Orthogonal amino acid reporters allow the selective labeling of different cell types in heterogeneous populations through the expression of engineered aminoacyl tRNA synthetases. Here, we demonstrate that para-ethynylphenylalanine (PEP) can be used as an orthogonal amino acid reporter for efficient selective labeling of an intracellular bacterial pathogen during infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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