Probiotic Potential and Antimicrobial Activity of Enterococcus faecium Isolated from Chicken Caecal and Fecal Samples

Autor: Hafsa, Salma H. Abu, A. Mendonca, B. Brehm-Stecher, A. A. Hassan, S. A. Ibrahim
Jazyk: angličtina
Rok vydání: 2015
Předmět:
DOI: 10.5281/zenodo.1100142
Popis: Enterococci are important inhabitants of the animal intestine and are widely used in probiotic products. A probiotic strain is expected to possess several desirable properties in order to exert beneficial effects. Therefore, the objective of this study was to isolate, characterize and identify Enterococcus sp. from chicken cecal and fecal samples to determine potential probiotic properties. Enterococci were isolated from chicken ceca and feces of thirty three clinically healthy chickens from a local farm. In vitro studies were performed to assess antibacterial activity of the isolated LAB (using agar well diffusion and cell free supernatant broth technique against Salmonella enterica serotype Enteritidis), survival in acidic conditions, resistance to bile salts, and their survival during simulated gastric juice conditions at pH 2.5. Isolates were identified by biochemical carbohydrate fermentation patterns using an API 50 CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate belonging to E. faecium species exhibited inhibitory effect against S. enteritidis. This isolate producing a clear zone as large as 10.30 mm or greater and was able to grow in the coculture medium and at the same time, inhibited the growth S. enteritidis. In addition, E. faecium exhibited significant resistance under highly acidic conditions at pH 2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing ability to survive in the presence of simulated gastric juice at pH 2.5. Based on these results, E. faecium isolate fulfills some of the criteria to be considered as a probiotic strain and therefore, could be used as a feed additive with good potential for controlling S. Enteritidis in chickens. However, in vivo studies are needed to determine the safety of the strain.
{"references":["H. A. Abdel-Rahman., S. M. Shawky, H. Ouda, A. A. Nafeaa, and S. H.\nOrabi, \"Effect of Two Probiotics and Bioflavonoids Supplementation to\nthe Broilers Diet and Drinking Water on the Growth Performance and\nHepatic Antioxidant Parameters\". Global Veterinaria 10 (6): 734-741,\n2013.","R. Herich, and M. Levkut, \"Lactic acid bacteria, probiotics and immune\nsystem\". Veterinární medicína, 47, 169-180, 2002.","P. Hlivak, J. Odraska, M. Ferencik, L. Ebringer, E. Jahnova, and A.\nMikes, \"One-year application of probiotic strain Enterococcus faecium\nM-74 decreases serum cholesterol levels\". Bratisl. Lek. Listy 106, 67–\n72, 2005.","Y. Huang, and Y. Zheng, \"The probiotic Lactobacillus acidophilus\nreduces cholesterol absorption through the down-regulation of\nNiemann–Pick C1-like 1 in Caco-2 cells\". Br. J. Nutr. 9, 1–6, 2009.","L. Pascual, F. Ruiz, W. Giordano, and I. L. Barberis, \"Vaginal\ncolonization and activity of the probiotic bacterium Lactobacillus\nfermentum L23 in a murine model of vaginal tract infection\". Journal of\nMedical Microbiology 59, 360–364, 2010.","O. Ashayerizadeh, B. Dastar, M. Shams Shargh, E. Rahmatnejad, and A.\nAshayerizadeh, \"Influence of prebiotic and two herbal additives on\ninterior organs and hematological indices of broilers\". Journal of Animal\nand Veterinary Advances. 8(9), p. 1851-1855, 2009.","A. Bhardwaj, G. Kaur, H. Gupta, S. Vij, and R. K. Malik, \"Interspecies\ndiversity, safety and probiotic potential of bacteriocinogenic Enterococcus faecium isolated from dairy food and human faeces\".\nWorld Journal of Microbiology and Biotechnology, 27, 591-602, 2011.","J. A. Patterson, and K. M. Burkholder, \"Application of prebiotics and\nprobiotics in poultry production. Poultry Sci., 82: 627-631, 2003.","A. Ahmadova, S. D. Todorov, Y. Choiset, H. Rabesona, T. M. Zadi,\nKuliyev, A., B.D.G.M. Franco, J. M. Chobert, and T. Haertlé,\n\"Evaluation of antimicrobial activity, probiotic properties and safety of\nwild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal\ncheese\". Food Control 30, 631–64, 2013.\n[10] B. B. Matijašic´, T. Obermajer, and I. Rogelj, \"Quantification of\nLactobacillus gasseri, Enterococcus faecium and Bifidobacterium\ninfantis in a probiotic OTC drug by real-time PCR\". Food Control 21,\n419–425, 2010.\n[11] L. Saavedra, M. P. Taranto, F. Sesma, and G. F. de Valdez, \"Homemade\ntraditional cheeses for the isolation of probiotic Enterococcus faecium\nstrains\". Int. J. Food Microbiol. 88, 241–245, 2003.\n[12] J. Benyacoub, P. F. Perez, F. Rochat, K. Y. Saudan, G. Reuteler, N.\nAntille, M. Humen, G. L. De Antoni, C. Cavadini, S. Blum, and E. J.\nSchiffrin, \"Enterococcus faecium SF68 enhances the immune response\nto Giardia intestinalisin mice\". J. Nutr. 135: 1171–1176, 2005.\n[13] D. G. V. Emmanuel, A. Jafari, K. A. Beauchemin, J. A. Leedle, and B.\nN. Ametaj, \"Feeding live cultures of Enterococcus faecium and\nSaccharomyces cerevisiae induces an inflammatory response in feedlot\nsteers\". J. Anim. Sci. 85:233–239, 2007.\n[14] U. Lodemann, K. Hubener, N. Jansen, and H. Martens, \"Effects of\nEnterococcus faecium NCIMB 10415 as probiotic supplement on\nintestinal transport and barrier function of piglets\". Arch. Anim. Nutr.\n60:35–48, 2006.\n[15] L. Scharek, J. Guth, K. Reiter, K. D. Weyrauch, D. Taras, P. Schwerk, P.\nSchierack, M. F. Schmidt, L. H. Wieler, and K. Tedin, \"Influence of a\nprobiotic Enterococcus faecium strain on development of the immune\nsystem of sows and piglets\". Vet. Immunol. Immunopathol. 105:151–\n161, 2005.\n[16] W. Theppangna, K. Otsuki, and T. Murase, \"Inhibitory effects of\nEnterococcus strains obtained from a probiotic product on in vitro\ngrowth of Salmonella enteric serovar Enteritidis strain IFO3313\". J.\nFood Prot. 69: 2258–2262, 2006.\n[17] D. Taras, W. Vahjen, M.Macha, and O. Simon, \"Performance, diarrhea\nincidence, and occurrence of Escherichia coli virulence genes during\nlong-term administration of a probiotic Enterococcus faecium strain to\nsows and piglets\". J. Anim. Sci. 84:608–617, 2006.\n[18] M. Tokumaru, H. Konuma, M. Umesako, S.Konno, and K. Shinagawa,\n\"Rates of detection of Salmonella and Campyobacterin meats in\nresponse to the sample size and the infection level of each species\". Int.\nJ. Food Microbiol. 13:41–46, 1991.\n[19] M. A. Ehrmann, P. Kurzak, J. Bauer, and R. F. Vogel, \"Characterization\nof lactobacilli towards their use as probiotic adjuncts in poultry\". J.\nAppl. Microbiol. 92:966–975, 2002.\n[20] L. M. Shin, R. J. McNally, S. M. Kosslyn, W. L. Thompson, S. L.\nRauch, and N. M. Alpert, \"Regional cerebral blood flow duringscriptdriven\nimagery in childhood sexual abuse-related posttraumatic stress\ndisorder: A positron emission tomographic investigation\". Am J\nPsychiatry 156:575–584, 1999.\n[21] K. Perelmuter, M. Fraga, and P. Zunino, \"In vitro activity of potential\nprobiotic Lactobacillus murinus isolated from the dog\". Journal of\nApplied Microbiology, 104, 1718-1725, 2008.\n[22] Y. Huang, and M. C. Adams, \"In vitro assessment of the upper\ngastrointestinal tolerance of potential probiotic dairy propionibacteria\".\nInternational Journal of Food Microbiology, 91, 253 -260, 2004.\n[23] A. R. Madureira, M. S. Gião, M. E. Pintado, A. M. P. Gomes, C. Freitas,\nand F. X. Malcata, \"Incorporation and survival of probiotic bacteria in\nwhey cheese matrices\". J. Food Sci. 70(3): M161-M165, 2005.\n[24] M. Succi, P. Tremonte, A. Reale, E. Sorrentino, L. Grazia, and S.\nPacifico, \"Bile salt and acid tolerance of Lactobacillus rhamnosus\nstrains isolated from Parmigiano Reggiano cheese\". FEMS\nMicrobiology Letters, 244, 129-137, 2005.\n[25] S. Pieniz, R. Andreazza, T. Anghinoni, F. Camargo, and A. Brandelli,\n\"Probiotic potential, antimicrobial and antioxidant activities of\nEnterococcus durans strain LAB18s. Food Control 37, 251-256, 2014.\n[26] P. Sun, J. Wang, and Y. Jiang, \"Effects of Enterococcus faecium (SF68)\non immune function in mice\". Food Chemistry, 123, 63-68, 2010.\n[27] B. Hyronimus, C. L. Marrec, S. A. Hadj, and A. Deschamps, \"Acid and\nbile tolerance of spore-forming lactic acid bacteria\". International\nJournal of Food Microbiology, 61, 193-197, 2000.\n[28] J. Gong, R., J. Forster, and H. Yu, \"Diversity and phylogenetic analysis\nof bacteria in the mucosa of chicken ceca and comparison with bacteria\nin the cecal lumen\". FEMS Microbiol Lett 208:1–7, 2002.\n[29] J. B. Payne, J. A. Osborne, P. K. Jenkins, and B. W. Sheldon, \"Modeling\nthe growth and death kinetics of Salmonella in poultry litter as a\nfunction of pH and water activity\". Poult. Sci., 86: 191-201, 2007.\n[30] EFSA, The community summary report on trends and sources of\nzoonoses and zoonotic agents in the European Union in 2007. EFSA J\n223:1–320, 2009.\n[31] H. R. Taheri, H. Moravej, F. Tabandeh, M. Zaghari, and M. Shivazad,\n\"Screening of lactic acid bacteria toward their selection as a source of\nchicken probiotic\". Poult. Sci. 88:1586-1593, 2009.\n[32] M. Chichlowski, J. Croom, B. W. McBride, G. Davis, L. Daniel, and M.\nKoci, \"Direct-fed microbial and salinomycin modulate whole body and\nintestinal oxygen consumption and intestinal enterocytes cytokine\nproduction in the broiler chick\". Poult. Sci., 86: 1100-1106, 2007.\n[33] M. A. Riley, and J. E. Wertz, \"Bacteriocins: evolution, ecology, and\napplication\". Annual review of microbiology, Vol.56, pp. 117-137, ISSN\n0066-4227, 2002.\n[34] P. Audisio, A.De Biase, G. Antonini, M. Belfiore, and M. Oliverio,\n\"Morphological, molecular and ecological evidence of a new Euro-\nAnatolian species of the Meligethes coracinus complex (Coleoptera:\nNitidulidae). Insect Systematics and Evolution, Volume 31, Issue\n4, p. 361 – 385, 2000.\n[35] R. Herich, T. Kokinčáková, A. Lauková, and M. Levkutová, \"Effect of\npreventive application of Enterococcus faecium EF55 on intestinal\nmucosa during salmonellosis in Chickens\". Czech J. Anim. Sci., 55, (1):\n42–47, 2010.\n[36] V. Strompfová, and A. Lauková, \"In vitro study on bacteriocin\nproduction of enterococci associated with chickens\". Anaerobe 13: 228-\n237, 2007.\n[37] M. Ashraf, and N. A. Akram, \"Improving salinity tolerance of plants\nthrough conventional breeding and genetic engineering: an analytical\ncomparison\". Biotechnol. Adv., 27: 744-52, 2009."]}
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