NOX1 mediates metabolic heart disease in mice and is upregulated in monocytes of humans with diastolic dysfunction
| Autor: | Melania Balzarolo, Giacomo Della Verde, Otmar Pfister, Lydia Joray, Emma L. Robinson, Saskia C.A. de Jager, Hester M. den Ruijter, Gideon B Valstar, Stephane Heymans, Vera Lorenz, Michika Mochizuki, Beat A. Kaufmann, Gabriela M. Kuster, Lifen Xu |
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| Přispěvatelé: | RS: Carim - H02 Cardiomyopathy, Cardiologie, MUMC+: MA Med Staf Spec Cardiologie (9) |
| Rok vydání: | 2021 |
| Předmět: |
CD31
Male Lipopolysaccharides medicine.medical_specialty Heart disease Heart Diseases Physiology GP91(PHOX) HOMOLOGS Inflammation BLOOD-PRESSURE Monocytes Endothelial activation Pathogenesis ACTIVATION Mice Immune system NADPH OXIDASES Metabolic Diseases Physiology (medical) Internal medicine NOX1 DIABETIC CARDIOMYOPATHY Medicine FAILURE Humans Animals Metabolic heart disease RESIDENT CARDIAC MACROPHAGES Mice Knockout business.industry Endothelial Cells metabolic heart disease Hypertrophy medicine.disease Mice Inbred C57BL Endocrinology PRESERVED EJECTION FRACTION inflammation ENDOTHELIAL DYSFUNCTION cardiovascular system Diastolic dysfunction medicine.symptom Metabolic syndrome Cardiology and Cardiovascular Medicine Heart failure with preserved ejection fraction business hypertrophy |
| Zdroj: | Cardiovascular Research, 118(14), 2973-2984. Oxford University Press |
| ISSN: | 1755-3245 0008-6363 |
| Popis: | AIMS: Microvascular inflammation plays an important role in the pathogenesis of diastolic dysfunction (DD) and metabolic heart disease. NOX1 is expressed in vascular and immune cells and has been implicated in the vascular pathology of metabolic disease. However, its contribution to metabolic heart disease is less understood.METHODS AND RESULTS: NOX1-deficient mice (KO) and male wild-type (WT) littermates were fed a high-fat high-sucrose diet (HFHS) and injected streptozotocin (75 mg/kg i.p.) or control diet (CTD) and sodium citrate. Despite similar weight gain and increase in fasting blood glucose and insulin, only WT-HFHS but not KO-HFHS mice developed concentric cardiac hypertrophy and elevated left ventricular filling pressure. This was associated with increased endothelial adhesion molecule expression, accumulation of Mac-2-, IL-1β- and NLRP3-positive cells and nitrosative stress in WT-HFHS but not KO-HFHS hearts. Nox1 mRNA was solidly expressed in CD45+ immune cells isolated from healthy mouse hearts, but was negligible in cardiac CD31+ endothelial cells. However, in vitro, Nox1 expression increased in response to LPS in endothelial cells and contributed to LPS-induced upregulation of Icam-1. Nox1 was also upregulated in mouse bone marrow-derived macrophages in response to LPS. In peripheral monocytes from age- and sex-matched symptomatic patients with and without DD, NOX1 was significantly higher in patients with DD compared to those without DD.CONCLUSIONS: NOX1 mediates endothelial activation and contributes to myocardial inflammation and remodeling in metabolic disease in mice. Given its high expression in monocytes of humans with DD, NOX1 may represent a potential target to mitigate heart disease associated with DD.TRANSLATIONAL PERSPECTIVE: In their multifactorial pathogenesis, diastolic dysfunction (DD) and heart failure with preserved ejection fraction (HFpEF) still remain poorly understood. They frequently occur in patients with obesity and metabolic syndrome. Microvascular inflammation and dysfunction have recently been recognized as major driving forces. We show that genetic deletion of Nox1 prevents cardiac inflammation, remodeling and dysfunction in metabolic disease in mice and find NOX1 upregulated in peripheral monocytes of patients with DD. These findings add to our understanding how obesity, inflammation and heart disease are linked, which is a prerequisite to find therapeutic strategies beyond the control of co-morbidities in HFpEF. |
| Databáze: | OpenAIRE |
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