First Signal from a Broadband Cryogenic Preamplifier Cooled by Circulating Liquid Nitrogen in a 7 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
| Autor: | SeungYong Kim, Kyungjae Lee, Hyun Sik Kim, Se Gyu Lee, Yeon Suk Choi, Myoung Choul Choi, Jeong Min Lee, Sang Hwan Choi, Stefan Karl-Heinz Stahl |
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| Rok vydání: | 2012 |
| Předmět: |
Fourier Analysis
Nitrogen Preamplifier Chemistry Cyclotron Analytical chemistry Cyclotrons Liquid nitrogen Mass spectrometry Mass Spectrometry Fourier transform ion cyclotron resonance Analytical Chemistry law.invention Cold Temperature symbols.namesake Fourier transform law Spectroscopy Fourier Transform Infrared symbols Water cooling Hybrid mass spectrometer |
| Zdroj: | Analytical Chemistry. 84:10543-10548 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Despite the outstanding performance of Fourier transform ion cyclotron/mass spectrometry (FTICR/MS), the complexity of the cellular proteome or natural compounds presents considerable challenges. Sensitivity is a key performance parameter of a FTICR mass spectrometer. By improving this parameter, the dynamic range of the instrument can be increased to improve the detection signal of low-abundance compounds or fragment ion peaks. In order to improve sensitivity, a cryogenic detection system was developed by the KBSI (Korean Basic Science Institute) in collaboration with Stahl-Electronics (Mettenheim, Germany). A simple, efficient liquid circulation cooling system was designed and a cryogenic preamplifier implemented inside a FTICR mass spectrometer. This cooling system circulates a cryoliquid from a Dewar to the "liquid circulation unit" through a CF flange to cool a copper block and a cryopreamplifier; the cooling medium is subsequently exhausted into the air. The cryopreamplifier can be operated over a very wide temperature range, from room temperature to low temperature environments (4.2 K). First, ion signals detected by the cryopreamplifier using a circulating liquid nitrogen cooling system were observed and showed a signal-to-noise ratio (S/N) about 130% better than that obtained at room temperature. |
| Databáze: | OpenAIRE |
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