Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin
| Autor: | Goran Zgrablić, Leo Frkanec, Daniel Mark Lyons, Željko Jakšić, Petra Burić, Filip Stipić, Galja Pletikapić, Maja Dutour Sikirić |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Bioluminescence Resonance Energy Transfer Techniques
Fluorophore Analytical chemistry Nanoparticle Metal Nanoparticles Biosensing Techniques Photochemistry 7. Clean energy Lanthanoid Series Elements Antibodies chemistry.chemical_compound Colloid and Surface Chemistry Okadaic Acid Animals Humans Shellfish Poisoning Colloids Physical and Theoretical Chemistry Particle Size Fluorescent Dyes Detection limit chemistry.chemical_classification Chemistry Biomolecule Surfaces and Interfaces General Medicine Okadaic acid Bivalvia Biosensor diagnostic nanoparticle lanthanide immunoassay point-of-care Nanoparticles Marine Toxins Luminescence Marine toxin Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces |
| Popis: | With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore: nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37-3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap, and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care. |
| Databáze: | OpenAIRE |
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