Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks
| Autor: | Qin-Ming Fan, Wen-Feng He, Yu-Zhu Shi, Yu-Qiong Hu, Ding-Ping Bai, Ang Li, Yue Chen, Ru-Tang Luo |
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| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system Candidate gene Gonad lcsh:QH426-470 lcsh:Biotechnology MAP3K1 Biology Sex differentiation Andrology Transcriptome 03 medical and health sciences 0302 clinical medicine lcsh:TP248.13-248.65 Genetics medicine Animals Gene Regulatory Networks KEGG Gonads Gene 030304 developmental biology Sex Characteristics 0303 health sciences Sexual differentiation RSPO3 Sequence Analysis RNA Gene Expression Profiling Gene Expression Regulation Developmental Cell Differentiation lcsh:Genetics Ducks medicine.anatomical_structure 030220 oncology & carcinogenesis Sex-related genes Female Muscovy ducks Research Article Biotechnology |
| Zdroj: | BMC Genomics BMC Genomics, Vol 21, Iss 1, Pp 1-17 (2020) |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-020-06852-z |
| Popis: | Background Sex-related genes play a crucial role in gonadal differentiation into testes or ovaries. However, the genetic control of gonadal differentiation in Muscovy ducks remains unknown. Therefore, the objective of our study was to screen new candidate genes associated with ovarian and testicular development. Results In this study, 24 males before gonadal differentiation (MB), 24 females before gonadal differentiation (FB), 24 males after gonadal differentiation (MA) and 24 females after gonadal differentiation (FA) were selected from Putian Muscovy ducks, forming 4 groups. RNA-Seq revealed 101.76 Gb of clean reads and 2800 differentially expressed genes (DEGs), including 46 in MB vs FB, 609 in MA vs FA, 1027 in FA vs FB, and 1118 in MA vs MB. A total of 146 signalling pathways were enriched by KEGG analysis, among which 20, 108, 108 and 116 signalling pathways were obtained in MB vs FB, MA vs MB, MA vs FA and FA vs FB, respectively. In further GO and KEGG analyses, a total of 21 candidate genes related to gonad differentiation and development in Muscovy ducks were screened. Among these, 9 genes were involved in the differentiation and development of the testes, and 12 genes were involved in the differentiation and development of the ovaries. In addition, RNA-Seq data revealed 2744 novel genes. Conclusions RNA-Seq data revealed 21 genes related to gonadal differentiation and development in Muscovy ducks. We further identified 12 genes, namely, WNT5B, HTRA3, RSPO3, BMP3, HNRNPK, NIPBL, CREB3L4, DKK3, UBE2R2, UBPL3KCMF1, ANXA2, and OSR1, involved in the differentiation and development of ovaries. Moreover, 9 genes, namely, TTN, ATP5A1, DMRT1, DMRT3, AMH, MAP3K1, PIK3R1, AGT and ADAMTSL1, were related to the differentiation and development of testes. Moreover, after gonadal differentiation, DMRT3, AMH, PIK3R1, ADAMTSL1, AGT and TTN were specifically highly expressed in males. WNT5B, ANXA2 and OSR1 were specifically highly expressed in females. These results provide valuable information for studies on the sex control of Muscovy ducks and reveal novel candidate genes for the differentiation and development of testes and ovaries. |
| Databáze: | OpenAIRE |
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