Quantitative analysis of vesicle recycling at the calyx of Held synapse
| Autor: | Xufeng Qiu, Qianwen Zhu, Jianyuan Sun |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Auditory Pathways
Models Neurological Kinetics Presynaptic Terminals Neurotransmission Biology Synaptic Transmission Synaptic vesicle Synapse Microscopy Electron Transmission Animals Multidisciplinary Vesicle Excitatory Postsynaptic Potentials Biological Sciences Electric Stimulation Mice Inbred C57BL Microscopy Fluorescence Multiphoton Synapses Biophysics Excitatory postsynaptic potential Sensorimotor Cortex Synaptic Vesicles Synaptic signaling Neuroscience Calyx of Held Algorithms |
| Zdroj: | Proceedings of the National Academy of Sciences. 112:4779-4784 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1424597112 |
| Popis: | Vesicle recycling is pivotal for maintaining reliable synaptic signaling, but its basic properties remain poorly understood. Here, we developed an approach to quantitatively analyze the kinetics of vesicle recycling with exquisite signal and temporal resolution at the calyx of Held synapse. The combination of this electrophysiological approach with electron microscopy revealed that ∼80% of vesicles (∼270,000 out of ∼330,000) in the nerve terminal are involved in recycling. Under sustained stimulation, recycled vesicles start to be reused in tens of seconds when ∼47% of the preserved vesicles in the recycling pool (RP) are depleted. The heterogeneity of vesicle recycling as well as two kinetic components of RP depletion revealed the existence of a replenishable pool of vesicles before the priming stage and led to a realistic kinetic model that assesses the size of the subpools of the RP. Thus, our study quantified the kinetics of vesicle recycling and kinetically dissected the whole vesicle pool in the calyceal terminal into the readily releasable pool (∼0.6%), the readily priming pool (∼46%), the premature pool (∼33%), and the resting pool (∼20%). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|