Requirement of CD4-positive T cells for cellular recruitment to the lungs of mice in response to a particulate intratracheal antigen
| Autor: | H B Kaltreider, P K Byrd, Jeffrey L. Curtis, M L Warnock |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
CD4-Positive T-Lymphocytes
T cell CD8 Antigens Mice Nude Inflammation Immunoglobulin Fab Fragments Mice Immune system Antigen medicine Animals Antigens Lung Injections Spinal medicine.diagnostic_test biology Macrophages Antibodies Monoclonal General Medicine T lymphocyte Mice Inbred C57BL Bronchoalveolar lavage medicine.anatomical_structure Immunology biology.protein Cytokines Female medicine.symptom Antibody CD8 Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | To determine whether CD4+ T cells participate in the recruitment of other lymphocyte subsets to the lungs, we examined pulmonary immune responses in C57BL/6 mice treated in vivo with the MAb GK1.5, either intact (which depletes CD4+ cells) or as F(ab')2 fragments (which block CD4 molecules). After intratracheal challenge with sheep erythrocytes, antigen-primed mice treated with intact GK1.5 had marked decreases in lymphocytes and macrophages in bronchoalveolar lavage fluid and minimal parenchymal inflammation, compared to primed mice treated with an isotype-matched irrelevant antibody or with no antibody. At 7 d after challenge, flow cytometric analysis showed that numbers of Thy 1.2+ and B220+ cells, but not of CD8+ cells, were markedly decreased in lavage fluid of CD4-depleted mice. Similar suppression of the pulmonary immune response to intratracheal challenge was found in primed mice injected repeatedly with F(ab')2 fragments of GK1.5, which did not deplete CD4+ T cells, and in athymic mice. These findings indicate that, in response to a single intratracheal antigen challenge, recruitment to the lungs of leukocytes other than CD8+ T cells depends largely on CD4+ T cells, possibly because of signals requiring T cell activation via interactions with antigen-presenting cells. |
| Databáze: | OpenAIRE |
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