Long-term effects of somatostatin analogues in rat GH-secreting pituitary tumor cell lines
| Autor: | Germano Gaudenzi, Davide Saronni, Giovanni Vitale, Alessandra Dicitore, Maria Celeste Cantone, Luca Persani, Maria Orietta Borghi, S. Carra |
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| Rok vydání: | 2021 |
| Předmět: |
Adenoma
Embryo Nonmammalian Time Factors Endocrinology Diabetes and Metabolism Apoptosis 030209 endocrinology & metabolism Octreotide Peptides Cyclic Animals Genetically Modified 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Acromegaly Tumor Cells Cultured medicine Animals Propidium iodide Viability assay Zebrafish Long-term treatment Pituitary tumors Cell cycle medicine.disease Somatotrophs Growth hormone secretion Rats Somatostatin chemistry Somatostatin analogs 030220 oncology & carcinogenesis Cancer research Original Article GH-secreting pituitary tumor Growth Hormone-Secreting Pituitary Adenoma |
| Zdroj: | Journal of Endocrinological Investigation |
| ISSN: | 1720-8386 |
| DOI: | 10.1007/s40618-021-01609-1 |
| Popis: | Purpose First-generation somatostatin analogs, octreotide (OCT) and lanreotide, are the cornerstone for the medical treatment of growth hormone (GH)-secreting pituitary tumors. A new multireceptor analog, such as pasireotide (PAS), showed better activity than OCT in long-term treatment of patients with acromegaly, but modulation of intracellular key processes is still unclear in vitro. In this study, we evaluated the antitumor activity of OCT and PAS in two GH-secreting pituitary tumor cell lines, GH3 and GH4C1, after a long-term incubation. Methods The effects of PAS and OCT on the cell viability, cell cycle, apoptosis, GH secretion, and tumor-induced angiogenesis have been evaluated through a colorimetric method (MTS Assay), DNA flow cytometry with propidium iodide, and Annexin V-FITC/propidium iodide staining, ELISA assay and zebrafish platform, respectively. Results PAS showed a more potent antitumor activity compared to OCT in GH3 cell line exerted through inhibition of cell viability, perturbation of cell cycle progression, and induction of apoptosis after 6 days of incubation. A concomitant decrease in GH secretion has been observed after 2 days of incubation only with PAS. No effect on tumor-induced angiogenesis has been reported after treatment with OCT or PAS in zebrafish/tumor xenograft model. Conclusion Long-term incubation with PAS showed a more potent antitumor activity than that reported after OCT in GH3 cells, mainly modulated by a cell cycle perturbation and a relevant induction in apoptosis. |
| Databáze: | OpenAIRE |
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