| Autor: |
Karen León Arcia, Heidi Quintero Álvarez, Laura María Pantoja-Echevarría, Yainelis Garrido Nicot |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of microbiological methods. 195 |
| ISSN: |
1872-8359 |
| Popis: |
Genomic DNA preparation is a critical step for successful fingerprinting analysis by Pulsed Field Gel Electrophoresis (PFGE). This paper presents a simple and rapid protocol to prepare DNA samples from up to 24 bacterial isolates simultaneously. It involves performing the conventional PFGE sample preparation steps (cell growth and harvest, agarose-immobilization of intact cells, and DNA release and purification) into a 24-wells culture plate, without subjecting the biological material to repeated transference of containers. The single-container protocol rendered high quality genomic DNA from E. coli clinical isolates. The DNA yields obtained from samples prepared with the single-container protocol showed no differences to those obtained using the conventional DNA sample preparation for PFGE. This procedure is a cost-effective alternative that provides a larger capacity of analysis to the PFGE technique, which could be advantageous in a context of infectious disease outbreaks for pathogens molecular subtyping. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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