Is t(14;18)(q32;q21) a constant finding in follicular lymphoma? An interphase FISH study on 63 patients
| Autor: | Hervé Avet-Loiseau, L Baranger-Papot, Anne-Sophie Moreau, Marc Zandecki, Franck Geneviève, Alban Godon, Noel-Jean Milpied, Pascaline Talmant |
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| Rok vydání: | 2003 |
| Předmět: |
Chromosomes
Artificial Bacterial Cancer Research Pathology medicine.medical_specialty Follicular lymphoma Chromosomal translocation Biology Translocation Genetic Fusion gene Internal medicine medicine Humans Interphase Lymphoma Follicular In Situ Hybridization Fluorescence Retrospective Studies Chromosomes Human Pair 14 Gene Rearrangement Ploidies Hematology medicine.diagnostic_test DNA Neoplasm Gene rearrangement medicine.disease Chromosome Banding Lymphoma Proto-Oncogene Proteins c-bcl-2 Oncology Karyotyping France Lymphoma Large B-Cell Diffuse Chromosomes Human Pair 18 DNA Probes Immunoglobulin Heavy Chains Fluorescence in situ hybridization |
| Zdroj: | Leukemia. 17:255-259 |
| ISSN: | 1476-5551 0887-6924 |
| DOI: | 10.1038/sj.leu.2402739 |
| Popis: | The translocation (14;18)(q32;q21) is the hallmark of follicular lymphoma (FL). However, conventional cytogenetics and PCR techniques fail to detect it in at least 10% of cases. In order to evaluate the true incidence of this translocation in FL, we analyzed 63 patients with FL, and 17 patients with diffuse large cell lymphoma (DLCL) corresponding to suspected FL transformations using interphase fluorescence in situ hybridization (FISH). Colocalized signals related to the translocation were observed in 19-92% of cells (median = 51%), corresponding to positivity over the threshold in all (63/63) cases. Similarly, 16/17 possibly secondary DLCL displayed the translocation. Although some cytogenetic changes might be missed by this FISH assay (such as rare insertion, or translocations with other chromosomal partners), our results stress t(14;18)(q32;q21) as an almost constant finding in FL. Our sensitive interphase FISH assay should be of great value to define FL more accurately, namely in patients included into therapeutic trials. Furthermore, this approach could be of interest in (re)defining some types of FL, especially the grade 3 FL which frequently lack t(14;18). |
| Databáze: | OpenAIRE |
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