Preparation of Electrode Modified with Methanobactin Functionalized Gold Nanoparticle and Mimic Superoxide Dismutase Activity
| Autor: | Ke Han, Wei Li, Zhen-Xing Wang, Lin-Lin Chen, Bo-Xin Dou, Jia-Ying Xin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Superoxide Dismutase Superoxide Inorganic chemistry Imidazoles Biomedical Engineering Metal Nanoparticles Bioengineering Biosensing Techniques Electrochemical Techniques General Chemistry Methanobactin Condensed Matter Physics Electrochemistry Redox Dielectric spectroscopy chemistry.chemical_compound chemistry Colloidal gold Electrode General Materials Science Gold Cyclic voltammetry Electrodes Oligopeptides |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 19:7967-7973 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2019.16939 |
| Popis: | Using a mixed self-assembly method, this research utilized modified Mb-functionalized gold nanoparticles (Mb-AuNPs-MPA) on the gold electrode surface to prepare a biosensor which was applied to detect superoxide anion-free electron mediators. Together with the study on the performance of the sensor, the characteristics of modified nanoclusters were investigated by UV-Vis and FTIR and the electrochemical characteristics of the electrode surface were investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), respectively. It was demonstrated that the charge transfer resistance (Rct) of the modified electrode (Mb-MPA-AuNPs/Au) was 7862 Ω, the exchange current density (i0) was 32.7 μA/cm-2. And a pair of reversible and symmetrical redox peaks appeared after the coordination of Cu2+, and the electrical signal response of Cu2+/Mb-AuNPs-MPA/Au reached the highest. The superoxide anion generated in the basic DMSO system was determined by CV using the modified electrode Cu2+/Mb-AuNPs-MPA/Au. It was discovered that the superoxide anion had a strong disproportionation effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|