Repurposing an atherosclerosis targeting peptide for tumor imaging
| Autor: | Luciana Kovacs, Ryan A. Davis, Tanushree Ganguly, Roger Chammas, Julie L. Sutcliffe |
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| Rok vydání: | 2022 |
| Předmět: |
THP-1 Cells
Antigens Differentiation Myelomonocytic Fluorescent Antibody Technique Receptors Cell Surface Stroma RM1-950 Mice Antigens CD Neoplasms Animals Humans Fluorescent Dyes Receptors Scavenger Pharmacology Tumor Macrophages Optical Imaging technology industry and agriculture General Medicine Carbocyanines Atherosclerosis Immunohistochemistry Plaque Atherosclerotic Sulfo-Cy5 Disease Models Animal Peptide Therapeutics. Pharmacology Peptides Protein Binding |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 145, Iss, Pp 112469-(2022) |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2021.112469 |
| Popis: | Cancer and atherosclerosis are chronic diseases that share common characteristics at both early and advanced stages and can arise from multiple factors. Both diseases are characterized by uncontrolled cell proliferation, inflammation, angiogenesis and apoptosis. Herein we investigated the ability of a peptide (CTHRSSVVC), that was previously reported to bind atherosclerotic lesions to home in the tumor microenvironment. The CTHRSSVVC peptide was synthesized on solid phase and N-terminally labeled with a sulfo-Cy5 dye. The specific binding to macrophage was evaluated in vitro with flow cytometry and immunofluorescence and in vivo for tumor targeting in BALB/c mice bearing a 4T1 tumor using optical imaging. The sulfo-Cy5-CTHRSSVVC peptide was synthesized in greater than 99% purity. No selective binding of the sulfo-Cy5-CTHRSSVVC peptide to macrophages in vitro was observed, however in vivo the sulfo-Cy5-CTHRSSVVC peptide accumulated in the 4T1 tumor, with a tumor-to-normal tissue ratio of 7.21 ± 1.44 at 2 h post injection. Ex vivo analysis of tumor tissue by confocal microscopy suggested that the sulfo-Cy5-CTHRSSVVC peptide had accumulated in the stroma of the tumor specifically, in regions of spindle shaped cells. In conclusion, although the target for the sulfo-Cy5-CTHRSSVVC peptide remains to be identified, the Cy5-CTHRSSVVC peptide warrants further investigation as a tumor imaging agent. |
| Databáze: | OpenAIRE |
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