Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS
| Autor: | Henry A. Simila, David Kvaskoff, Pauline Ko, Darryl W. Eyles, Dallas R. English, Alicia K Heath |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Male Clinical Biochemistry Biology Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Signal strength Tandem Mass Spectrometry Lc ms ms Vitamin D and neurology Humans Vitamin D Dried blood Actual use Dried Blood Spot Testing Vitamin D metabolism Chromatography Spots Biochemistry (medical) Reference Standards 030104 developmental biology Calibration 030217 neurology & neurosurgery Chromatography Liquid |
| Zdroj: | Clinical chemistry. 62(4) |
| ISSN: | 1530-8561 |
| Popis: | BACKGROUND The noncalcemic actions of vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for vitamin D screening. In particular, there is intense interest in the impact of vitamin D on a variety of developmental conditions. The most readily accessible pediatric samples are dried blood spots, and health organizations are increasingly archiving such samples for later assessment of the antecedents of disease. METHODS In 2009, we developed a method to quantify the major circulatory form of vitamin D, 25-hydroxyvitamin D, in archived dried blood spots. Over the last 6 years, we have made substantial alterations to the published method to enhance throughput, sensitivity, and assay robustness. RESULTS With the alterations, the assay was 3 times faster than the previously published assay and had a >10-fold increase in signal strength. Intraassay imprecision decreased from 13.4% to 6.9%, and there was a 5-fold reduction in interfering phospholipids. In actual use over 2 years, the assay showed an interassay imprecision of 11.6%. CONCLUSIONS This assay has performed reliably over the past 6 years. The practical changes we have made should allow clinical chemists to successfully adapt this method. |
| Databáze: | OpenAIRE |
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