Bacteriocin-like inhibitory substance in aquaculture: a classic method of protein precipitation for a new aplicability
| Autor: | Marina Loures Borges, Rafhaela Tostes Fernandes, Dionéia Evangelista Cesar, Ana Carolina Morais Apolônio, Kelly Dutra Pacheco, Alessandro Del'Duca |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Bacilli
Salmonella food.ingredient Fish farming Gram-positive bacteria tilapia culture lcsh:QR1-502 medicine.disease_cause General Biochemistry Genetics and Molecular Biology bacteriocins lcsh:Microbiology food Bacteriocin medicine Food science lcsh:QH301-705.5 Ammonium sulfate precipitation biology Chemistry Tilapia biology.organism_classification bacteriocin-like inhibitory substance purification Aeromonas hydrophila Gram-positive bacilli lcsh:Biology (General) General Agricultural and Biological Sciences gram-positive bacilli |
| Zdroj: | Acta Scientiarum : Biological Sciences, Vol 40, Pp e37881-e37881 (2018) Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e37881 Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e37881 Acta Scientiarum Biological Sciences Universidade Estadual de Maringá (UEM) instacron:UEM |
| ISSN: | 1679-9283 1807-863X |
| Popis: | Techniques to decrease losses from bacterial diseases are always important to improve the fish production. The use of antagonistic substances (bacteriocins) has been proven to be a viable option. The aim of this study was to evaluate different methods of purification for bacteriocin like inhibitory substances (BLIS). For the purification process, we isolated and used two Gram-positive bacilli that produce antagonistic substances for pathogens in aquaculture. Tests for detection of interfering factors were also performed. After the confirmation that the antagonistic action was due the BLIS activity, we carried out the purification methods. The methods tested were: cell free supernatant, acid extraction and ammonium sulfate precipitation at two concentrations (20 and 50%). Salmonella Tiphy CFP/IAL1472 and Aeromonas hydrophila (isolated in a tilapia production environment) were used as indicators of the efficiency of extracts in controlling pathogenic potentials. A mmonium sulfate precipitation at 50% was the most appropriate for purifying the antagonistic substance for both indicators. The extracts of the two isolates remained active for 22 days at 25oC. These are promising results regarding the water and fish health without the use of antibiotics, in this manner being a safer environmental practice. |
| Databáze: | OpenAIRE |
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