Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani
| Autor: | Xu Liang, Ruiqing Song, Xiaoshuang Song, Xun Deng, Saiyaremu Halifu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Trichoderma virens ZT05 Plant Science antifungal effect of metabolites 01 natural sciences Article Microbiology Rhizoctonia solani Superoxide dismutase 03 medical and health sciences transcriptome sequencing analysis lcsh:Botany Ecology Evolution Behavior and Systematics Mycelium Ecology biology Antibiosis food and beverages biology.organism_classification bacterial infections and mycoses equipment and supplies Enzyme assay antagonism lcsh:QK1-989 030104 developmental biology Trichoderma biology.protein Antagonism 010606 plant biology & botany Peroxidase |
| Zdroj: | Plants Volume 9 Issue 7 Plants, Vol 9, Iss 912, p 912 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9070912 |
| Popis: | Trichoderma is a filamentous fungus that is widely distributed in nature. As a biological control agent of agricultural pests, Trichoderma species have been widely studied in recent years. This study aimed to understand the inhibitory mechanism of Trichoderma virens ZT05 on Rhizoctonia solani through the side-by-side culture of T. virens ZT05 and R. solani. To this end, we investigated the effect of volatile and nonvolatile metabolites of T. virens ZT05 on the mycelium growth and enzyme activity of R. solani and analyzed transcriptome data collected from side-by-side culture. T. virens ZT05 has a significant antagonistic effect against R. solani. The mycelium of T. virens ZT05 spirally wraps around and penetrates the mycelium of R. solani and inhibits the growth of R. solani. The volatile and nonvolatile metabolites of T. virens ZT05 have significant inhibitory effects on the growth of R. solani. The nonvolatile metabolites of T. virens ZT05 significantly affect the mycelium proteins of R. solani, including catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), selenium-dependent glutathione peroxidase (GSH-Px), soluble proteins, and malondialdehyde (MDA). Twenty genes associated with hyperparasitism, including extracellular proteases, oligopeptide transporters, G-protein coupled receptors (GPCRs), chitinases, glucanases, and proteases were found to be upregulated during the antagonistic process between T. virens ZT05 and R. solani. Thirty genes related to antibiosis function, including tetracycline resistance proteins, reductases, the heat shock response, the oxidative stress response, ATP-binding cassette (ABC) efflux transporters, and multidrug resistance transporters, were found to be upregulated during the side-by-side culture of T. virens ZT05 and R. solani. T. virens ZT05 has a significant inhibitory effect on R. solani, and its mechanism of action is associated with hyperparasitism and antibiosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|