Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration
| Autor: | Bin Deng, Darcy E. Wagner, David M. Hoganson, Daniel J. Weiss, Joseph Platz, Takamaru Ashikaga, Zain Khalpey, Zachary D. Borg, Elice M. Brooks, Charles S. Parsons, Ying-Wai Lam, Nicholas R. Bonenfant, Michael J. DeSarno, Dino Sokocevic, Rachael A. Oldinski |
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| Rok vydání: | 2014 |
| Předmět: |
Lung Diseases
Materials science Infrared Rays Sus scrofa Biophysics Bioengineering Article Mass Spectrometry Biomaterials Extracellular matrix Tissue engineering Cadaver medicine Animals Humans Regeneration Lung Decellularization Tissue Engineering Tissue Scaffolds Regeneration (biology) Mesenchymal stem cell Endothelial Cells Epithelial Cells Mesenchymal Stem Cells Fibroblasts respiratory system In vitro Extracellular Matrix respiratory tract diseases Perfusion medicine.anatomical_structure Thermography Mechanics of Materials Ceramics and Composites Ex vivo Biomedical engineering |
| Zdroj: | Biomaterials. 35:2664-2679 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2013.11.078 |
| Popis: | Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼ 1-3 cm(3)) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold. |
| Databáze: | OpenAIRE |
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