Saccharomyces cerevisiae strain expressing a plant fatty acid desaturase produces polyunsaturated fatty acids and is susceptible to oxidative stress induced by lipid peroxidation
| Autor: | Willibald Wonisch, Georg Waeg, Tanja Matijevic, Ana Čipak, Sepp D. Kohlwein, Morana Zivkovic, Oksana Tehlivets, Neven Zarkovic, Emma J. Collinson, Ian W. Dawes, Meinhard Hasslacher |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Fatty Acid Desaturases
Paraquat Free Radicals Molecular Sequence Data Saccharomyces cerevisiae medicine.disease_cause Biochemistry Lipid peroxidation chemistry.chemical_compound tert-Butylhydroperoxide Lipid oxidation yeast lipid oxidation PUFA oxidative stress reactive oxygen species Physiology (medical) medicine Amino Acid Sequence Cloning Molecular Plant Proteins chemistry.chemical_classification Aldehydes biology Fatty acid Hydrogen Peroxide biology.organism_classification Yeast Oxidative Stress Fatty acid desaturase chemistry Fatty Acids Unsaturated biology.protein Hevea Lipid Peroxidation Oxidative stress Polyunsaturated fatty acid |
| Zdroj: | Free Radical Biology and Medicine. 40:897-906 |
| ISSN: | 0891-5849 |
| Popis: | Although oxygen is essential for aerobic organisms, it also forms potentially harmful reactive oxygen species. For its simplicity, easy manipulation, and cultivation conditions, yeast is used as an attractive model in oxidative stress research. However, lack of polyunsaturated fatty acids in yeast membranes makes yeast unsuitable for research in the field of lipid peroxidation. Therefore, we have constructed a yeast strain expressing a Delta12 desaturase gene from the tropical rubber tree, Hevea brasiliensis. This yeast strain expresses the heterologous desaturase in an active form and, consequently, produces Delta9/Delta12 polyunsaturated fatty acids under inducing conditions. The functional expression of the heterologous desaturase did not affect cellular morphology or growth, indicating no general adverse effect on cellular physiology. However, the presence of polyunsaturated fatty acids changed the yeast's sensitivity to oxidative stress induced by addition of paraquat, tert-butylhydroperoxide, and hydrogen peroxide. This difference in sensitivity to the latter was followed by the formation of 4-hydroxy-2-nonenal, one of the end products of linoleic fatty acid peroxidation, which is known to play a role in cell growth control and signaling. Here we show that this yeast strain conditionally expressing the Delta12 desaturase gene provides a novel and well-defined eukaryotic model in lipid peroxidation research. Its potential to investigate the molecular basis of responses to oxidative stress, in particular the involvement of reactive aldehydes derived from fatty acid peroxidation, especially 4-hydroxy-2-nonenal, will be addressed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect