Methodologies for preparation of prokaryotic extracts for cell-free expression systems
| Autor: | Zachary Z. Sun, Stephanie D Cole, Aleksandr E. Miklos, Abel C. Chiao, Matthew W. Lux |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
GFP Green Fluorescent Protein Cell-free extract Computer science OD Optical Density lcsh:Biotechnology Biomedical Engineering Cell free Computational biology 01 natural sciences Applied Microbiology and Biotechnology Article 03 medical and health sciences Synthetic biology Structural Biology 010608 biotechnology lcsh:TP248.13-248.65 Genetics Methods Cell-free expression lcsh:QH301-705.5 030304 developmental biology 0303 health sciences CFE Cell-Free Expression TFF Tangential Flow Filtration Cell function Expression (mathematics) lcsh:Biology (General) OD - Optical density Cell-free systems CFPS Cell-Free Protein Synthesis TXTL Transcription and Translation |
| Zdroj: | Synthetic and Systems Biotechnology Synthetic and Systems Biotechnology, Vol 5, Iss 4, Pp 252-267 (2020) |
| ISSN: | 2405-805X |
| Popis: | Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity of Escherichia coli genetic strains available and their corresponding utility. We then trace the history of cell-extract methodology over the past 20 years, showing key improvements that lower the entry level for new researchers. Next, we survey the rise of new prokaryotic cell-free systems, with associated methods, and the opportunities provided. Finally, we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|