Chikungunya Virus Sequences Across the First Epidemic in Nicaragua, 2014–2015
Autor: | Poornima Parameswaran, Chunling Wang, Diane Wu, Saira Saborio, Lionel Gresh, Angel Balmaseda, Andrew Fire, Eva Harris, Meghana Eswarappa |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Silent mutation DNA Complementary Aedes albopictus Genotype viruses Nicaragua Biology medicine.disease_cause Medical and Health Sciences Vaccine Related 03 medical and health sciences Phylogenetics Complementary Biodefense Tropical Medicine Virology Genetic variation Genetics medicine Humans Viral Chikungunya Epidemics Clade Phylogeny Phylogenetic tree Prevention Genetic Variation virus diseases DNA Articles biology.organism_classification Vector-Borne Diseases Emerging Infectious Diseases Good Health and Well Being 030104 developmental biology Infectious Diseases Population Surveillance RNA Chikungunya Fever RNA Viral Parasitology Chikungunya virus |
Zdroj: | The American journal of tropical medicine and hygiene, vol 94, iss 2 |
ISSN: | 1476-1645 0002-9637 |
Popis: | Chikungunya is caused by the mosquito-borne arthrogenic alphavirus, chikungunya virus (CHIKV). Chikungunya was introduced into the Americas in late 2013 and Nicaragua in mid-2014. Here, we sequenced five imported and 30 autochthonous Nicaraguan CHIKV from cases identified in the first epidemic in the country between August 2014 and April 2015. One full-length and two partial genomic sequences were obtained by deep sequencing; Sanger methodology yielded 33 E1 sequences from five imported and 28 autochthonous cases. Phylogenetic analysis indicates that Nicaraguan CHIKV all belonged to the Asian genotype, Caribbean clade. Moreover, E1 gene sequences revealed accumulation of mutations in later months of the epidemic, including four silent mutations in 11 autochthonous cases and three non-synonymous mutations in three autochthonous cases. No mutations contributing to increased transmissibility by Aedes albopictus were identified in the E1 gene. This represents the most comprehensive set of CHIKV sequences available from the Americas to date. |
Databáze: | OpenAIRE |
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