Alternol inhibits proliferation and induces apoptosis in mouse lymphocyte leukemia (L1210) cells
| Autor: | Shuo Geng, Bo Sun, Chang-Ling Li, Xia Liu, Zhao-Zhe Liu, Jin Zhu, Shu Liu |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Clinical Biochemistry
Blotting Western Antineoplastic Agents Apoptosis Biology Heterocyclic Compounds 4 or More Rings Mice Prophase medicine Animals Leukemia L1210 Molecular Biology Gene Cell Proliferation chemistry.chemical_classification Membrane potential Membrane Potential Mitochondrial Reactive oxygen species Caspase 8 Molecular Structure Caspase 3 Alternaria Depolarization Cell Biology General Medicine medicine.disease Flow Cytometry Caspase 9 Cell biology Leukemia chemistry Proto-Oncogene Proteins c-bcl-2 L1210 cells Reactive Oxygen Species |
| Zdroj: | Molecular and cellular biochemistry. 306(1-2) |
| ISSN: | 0300-8177 |
| Popis: | Alternol is a novel compound purified from the fermenting products by microorganisms named as Alternaria alternata var. monosporus from the bark of Yew. In this study, we tested its effect on mouse lymphocyte leukemia L1210 cells. Alternol was found to inhibit the proliferation and induce apoptosis in L1210 cells. When the cells were treated with Alternol, chromatin condensation and phosphatidylserine externalization were observed with the down-regulation of the pro-survival gene Bcl-2 and the activation of caspase-3, caspase-9, but not caspase-8. Moreover, exposure of cells to Alternol resulted in a significant increase in reactive oxygen species (ROS) and mitochondrial transmembrane potential (DeltaPsim) depolarization. Taken together, these results demonstrate that Alternol is a potent agent in inducing L1210 cells to apoptosis, which involve caspase activation and ROS generation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink