Direct nuclear magnetic resonance observation of odorant binding to mouse odorant receptor MOR244-3
| Autor: | Jessica L. Burger, Kavita M. Jeerage, Thomas J. Bruno |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Odorant binding Biophysics chemistry.chemical_element Sensory system Olfaction Ligands Receptors Odorant Biochemistry Cell Line Cell membrane 03 medical and health sciences Cyclooctanes Mice Nuclear magnetic resonance medicine Animals Humans Sulfhydryl Compounds Receptor Molecular Biology G protein-coupled receptor chemistry.chemical_classification Binding Sites Molecular Structure Cell Biology Sulfur 030104 developmental biology medicine.anatomical_structure chemistry Odorants Thiol |
| Zdroj: | Analytical biochemistry. 502 |
| ISSN: | 1096-0309 |
| Popis: | Mammals are able to perceive and differentiate a great number of structurally diverse odorants through the odorant's interaction with odorant receptors (ORs), proteins found within the cell membrane of olfactory sensory neurons. The natural gas industry has used human olfactory sensitivity to sulfur compounds (thiols, sulfides, etc.) to increase the safety of fuel gas transport, storage, and use through the odorization of this product. In the United States, mixtures of sulfur compounds are used, but the major constituent of odorant packages is 2-methylpropane-2-thiol, also known as tert-butyl mercaptan. It has been fundamentally challenging to understand olfaction and odorization due to the low affinity of odorous ligands to the ORs and the difficulty in expressing a sufficient number of OR proteins. Here, we directly observed the binding of tert-butyl mercaptan and another odiferous compound, cis-cyclooctene, to mouse OR MOR244-3 on living cells by saturation transfer difference (STD) nuclear magnetic resonance (NMR) spectroscopy. This effort lays the groundwork for resolving molecular mechanisms responsible for ligand binding and resulting signaling, which in turn will lead to a clearer understanding of odorant recognition and competition. |
| Databáze: | OpenAIRE |
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