Molecular cloning of hepatic 5-aminolevulinate synthase
| Autor: | Gopesh Srivastava, J.D. Brooker, Brian K. May, Byron A. Pirola, Andrew A. Hobbs, Iain A. Borthwick, W.H. Elliott |
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| Rok vydání: | 1984 |
| Předmět: |
Base pair
Chick Embryo Molecular cloning Biochemistry Complementary DNA Animals Northern blot Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Molecular mass ATP synthase biology cDNA library Nucleic Acid Hybridization DNA Dicarbethoxydihydrocollidine Molecular biology Liver Enzyme Induction Polyribosomes Protein Biosynthesis biology.protein Allylisopropylacetamide 5-Aminolevulinate Synthetase |
| Zdroj: | European journal of biochemistry. 144(1) |
| ISSN: | 0014-2956 |
| Popis: | Hepatic 5-aminolevulinate synthase was induced in chick embryos by administration of the porphyrinogenic drugs 2-allyl-2-isopropylacetamide and 3,5-diethoxycarbonyl-1,4-dihydrocollidine. A cDNA library was constructed from drug-induced liver mRNA and clones containing sequences coding for 5-aminolevulinate synthase were identified by hybrid-selected translation. The identity of these clones was confirmed by comparing DNA sequence data with the amino acid sequence of peptides from purified 5-aminolevulinate synthase. From Northern blot analysis the size of the mRNA for 5-aminolevulinate synthase was estimated to be 2800 base pairs, approximately 600 base pairs more than that required to code for the primary translation product of relative molecular mass 74000. |
| Databáze: | OpenAIRE |
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