Ovarian hormones induce de novo DNA methyltransferase expression in the Siberian hamster suprachiasmatic nucleus
| Autor: | Perry Barrett, Kenneth G. Onishi, Javier Tello, Chris Coyle, Tyler J. Stevenson, Federico Caso, Elisabetta Tolla |
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| Přispěvatelé: | University of St Andrews. School of Medicine, University of St Andrews. Centre for Biophotonics, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. Cellular Medicine Division |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
endocrine system Phodopus Endocrinology Diabetes and Metabolism DNMT3B Vasoactive intestinal peptide Hamster Estrous Cycle 030209 endocrinology & metabolism Biology DNA methyltransferase 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Endocrinology Oestrogen Circadian Clocks Internal medicine medicine Animals DNA (Cytosine-5-)-Methyltransferases Circadian rhythm QR180 Immunology Rhythmic epigenetics Endocrine and Autonomic Systems Suprachiasmatic nucleus Ovary Circadian DAS Methylation DNA Methylation Neurosecretory Systems Neuroendocrine DNA methylation embryonic structures RG Gynecology and obstetrics QR180 RC0321 Female Suprachiasmatic Nucleus RG RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Gonadal Hormones 030217 neurology & neurosurgery |
| Popis: | TJS thanks the British Society for Neuroendocrinology and the Society for Reproduction and Fertility for research project funds. PB acknowledges the Scottish Government for funding. Experiments investigated neuroanatomically localized changes in de novo DNA methyltransferase expression in the female Siberian hamster (Phodopus sungorus). The objectives were to identify the neuroendocrine substrates that exhibit rhythmic Dnmt3a and Dnmt3b expression across the oestrous cycle and examine the role of ovarian steroids. Hypothalamic Dnmt3a expression was observed to significantly increase during the transition from proestrous to oestrous. A single bolus injection of diethylstilbestrol (DES) and progesterone was sufficient to increase Dnmt3a cell numbers and Dnmt3b immunoreactive intensity in the suprachiasmatic nucleus (SCN). In vitro analyses using an embryonic rodent cell line revealed that DES was sufficient to induce Dnmt3b expression. Upregulating DNA methylation in vitro reduced expression of vasoactive intestinal polypeptide, Vip, and the circadian clock gene, Bmal1. Together, these data indicate that ovarian steroids drive de novo DNA methyltransferase expression in the mammalian suprachiasmatic nucleus and increased methylation may regulate genes involved in the circadian timing of oestrous: Vip and Bmal1. Overall, epigenetically mediated neuroendocrine reproductive events may reflect an evolutionarily ancient process involved in the timing of female fertility. Postprint |
| Databáze: | OpenAIRE |
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