High-resolution atmospheric pressure infrared laser desorption/ionization mass spectrometry imaging of biological tissue
| Autor: | Zheng Wang, Bernhard Spengler, Karl Christian Schäfer, Sabine Guenther, Carmen Paschke, Martin Köstler, Thorsten Schramm, Andreas Römpp, Arne Leisner |
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| Rok vydání: | 2013 |
| Předmět: |
Diagnostic Imaging
Matrix-assisted laser desorption electrospray ionization Infrared Rays Analytical chemistry Physics::Optics 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry Mass spectrometry imaging Mass Spectrometry Analytical Chemistry Atmospheric-pressure laser ionization Mice Animals Physics::Atomic Physics Phospholipids Laser spray ionization Ambient ionization Brain Chemistry Chemistry Lasers 010401 analytical chemistry Far-infrared laser Equipment Design Ion source 0104 chemical sciences Atmospheric Pressure |
| Zdroj: | Analytical and bioanalytical chemistry. 405(22) |
| ISSN: | 1618-2650 |
| Popis: | An atmospheric pressure laser desorption/ionization mass spectrometry imaging ion source has been developed that combines high spatial resolution and high mass resolution for the in situ analysis of biological tissue. The system is based on an infrared laser system working at 2.94 to 3.10 μm wavelength, employing a Nd:YAG laser-pumped optical parametrical oscillator. A Raman-shifted Nd:YAG laser system was also tested as an alternative irradiation source. A dedicated optical setup was used to focus the laser beam, coaxially with the ion optical axis and normal to the sample surface, to a spot size of 30 μm in diameter. No additional matrix was needed for laser desorption/ionization. A cooling stage was developed to reduce evaporation of physiological cell water. Ions were formed under atmospheric pressure and transferred by an extended heated capillary into the atmospheric pressure inlet of an orbital trapping mass spectrometer. Various phospholipid compounds were detected, identified, and imaged at a pixel resolution of up to 25 μm from mouse brain tissue sections. Mass accuracies of better than 2 ppm and a mass resolution of 30,000 at m/z = 400 were achieved for these measurements. |
| Databáze: | OpenAIRE |
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