Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites

Autor: Rodrigo T. Hernandes, Ivan Hong Jun Koh, Ana Maria Alvim Liberatore, Vanessa Sperandio, Cecilia M. Abe, Denise Yamamoto, Fabiano T. Romão, Rodrigo Barbosa de Souza, Tânia A. T. Gomes
Přispěvatelé: Universidade Federal de São Paulo (UNIFESP), Universidade Estadual Paulista (Unesp), Instituto Butantan, University of Texas Southwestern Medical Center
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Bacterial Diseases
0301 basic medicine
Cell Lines
lcsh:Medicine
medicine.disease_cause
Epithelium
Escherichia albertii
Electricity
Intestinal mucosa
Medicine and Health Sciences
Type III Secretion Systems
Electron Microscopy
Intestinal Mucosa
lcsh:Science
Cells
Cultured
Microscopy
Multidisciplinary
Bacterial Gastroenteritis
Virulence
Physics
Enterobacteriaceae Infections
Cell Differentiation
Intestinal epithelium
Gastroenteritis
Mutant Strains
Infectious Diseases
Shigellosis
Physical Sciences
Female
Biological Cultures
Scanning Electron Microscopy
Anatomy
Research Article
Neglected Tropical Diseases
Escherichia
Gastroenterology and Hepatology
Biology
Research and Analysis Methods
Cell Line
Bacterial genetics
Microbiology
03 medical and health sciences
Genetics
medicine
Animals
Humans
Enteropathogenic Escherichia coli
Adhesins
Bacterial
Escherichia coli
lcsh:R
Biology and Life Sciences
Tropical Diseases
biology.organism_classification
Rats
Gastrointestinal Tract
Bacterial adhesin
Biological Tissue
Enterocytes
030104 developmental biology
Mutation
Electrical Resistance
lcsh:Q
Caco-2 Cells
Digestive System
Developmental Biology
Zdroj: Repositório Institucional da UNIFESP
Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
PLoS ONE, Vol 12, Iss 2, p e0171385 (2017)
PLoS ONE
Popis: Made available in DSpace on 2018-12-11T17:09:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Diarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo. Departamento de Microbiologia Imunologia E Parasitologia Universidade Federal de São Paulo Escola Paulista de Medicina (UNIFESP-EPM) Departamento de Microbiologia E Imunologia Instituto de Biociência Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Departamento de Cirurgia Universidade Federal de São Paulo Escola Paulista de Medicina (UNIFESP-EPM) Laboratório de Biologia Celular Instituto Butantan Department of Microbiology and Biochemistry University of Texas Southwestern Medical Center Departamento de Microbiologia E Imunologia Instituto de Biociência Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) CAPES: AUX-PE-PNPD 2350/2011
Databáze: OpenAIRE
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