Molecular identification of an androgen receptor and the influence of long-term aggressive interaction on hypothalamic genes expression in black rockfish (Sebastes schlegelii)

Autor: Xin Sun, Xiumei Zhang, Qingqing Bai, Yuyang Zhang, Xiuwen Xu, Jianguang Qin
Rok vydání: 2021
Předmět:
Fish Proteins
Male
endocrine system
medicine.medical_specialty
Time Factors
Physiology
medicine.drug_class
030310 physiology
ved/biology.organism_classification_rank.species
Hypothalamus
Hypothalamic–pituitary–gonadal axis
Ovary
Gonadotropin-releasing hormone
Biology
Gonadotropin-Releasing Hormone
03 medical and health sciences
Behavioral Neuroscience
Sex Factors
0302 clinical medicine
Internal medicine
medicine
Animals
Testosterone
Ecology
Evolution
Behavior and Systematics
Black rockfish
0303 health sciences
Behavior
Animal
ved/biology
Reproduction
Androgen
biology.organism_classification
Perciformes
Aggression
Androgen receptor
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
Receptors
Androgen
Female
Animal Science and Zoology
Sebastes schlegelii
030217 neurology & neurosurgery
Zdroj: Journal of Comparative Physiology A. 207:401-413
ISSN: 1432-1351
0340-7594
DOI: 10.1007/s00359-021-01480-8
Popis: This study aims to explore the mechanism on how aggressive interaction alters reproductive physiology by testing whether aggressive interaction can activate the reproductive neuroendocrine function via the hypothalamus-pituitary-gonadal (HPG) axis in black rockfish (Sebastes schlegelii). The expressions of the androgen receptor gene (ar) and gonadotropin-releasing hormone genes (gnrhs), the concentration of plasma androgens, and GSI (the ratio of testes mass to body mass) were compared between the interaction group (dominant males or subordinate males) and the isolation group in male black rockfish after 3 weeks. A full-length cDNA encoding an androgen receptor (AR) of 766 amino acids was isolated. Transcripts encoding this AR were detected at a high relative abundance in the liver, kidney, testis, ovary, muscle, and intestine tissue. Further evaluation of brain genes transcripts abundance revealed that the mRNA levels of gnrh I and ar genes were significantly different between the interaction group and the isolation group in the hypothalamus. However, no significant difference was detected in testosterone, 11-keto-testosterone, and GSI between these two groups. This study indicates that a long-term aggressive interaction affect the expression of hypothalamic gnrh I and ar but may not change the physiological function of the HPG axis in an all-male condition.
Databáze: OpenAIRE
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