Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1
| Autor: | José L. Maestro, David Martín, Oscar Maestro, Ferran Borras-Castells, Claudia Nieva, Xavier Bellés |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, European Commission, Ministerio de Ciencia e Innovación (España), Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.medical_specialty media_common.quotation_subject Receptors Cytoplasmic and Nuclear Biology Steroidogenic Factor 1 01 natural sciences Article 03 medical and health sciences Corpora Allata Internal medicine medicine Juvenile Animals Protein Isoforms Metamorphosis Receptor media_common Cell Proliferation Multidisciplinary Reproduction Blattellidae Juvenile Hormones 010602 entomology 030104 developmental biology Endocrinology Nuclear receptor Juvenile hormone Insect Proteins Female Vitellogenesis Corpus allatum Hormone |
| Zdroj: | Scientific Reports Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | In insects, the transition from juvenile development to the adult stage is controlled by juvenile hormone (JH) synthesized from the corpora allata (CA) glands. Whereas a JH-free period during the last juvenile instar triggers metamorphosis and the end of the growth period, the reappearance of this hormone after the imaginal molt marks the onset of reproductive adulthood. Despite the importance of such transition, the regulatory mechanism that controls it remains mostly unknown. Here, using the hemimetabolous insect Blattella germanica, we show that nuclear hormone receptors Seven-up-B (BgSvp-B) and Fushi tarazu-factor 1 (BgFTZ-F1) have essential roles in the tissue-and stage-specific activation of adult CA JH-biosynthetic activity. Both factors are highly expressed in adult CA cells. Moreover, RNAi-knockdown of either BgSvp-B or BgFTZ-F1 results in adult animals with a complete block in two critical JH-dependent reproductive processes, vitellogenesis and oogenesis. We show that this reproductive blockage is the result of a dramatic impairment of JH biosynthesis, due to the CA-specific reduction in the expression of two key JH biosynthetic enzymes, 3-hydroxy-3-methylglutaryl coenzyme A synthase-1 (BgHMG-S1) and HMG-reductase (BgHMG-R). Our findings provide insights into the regulatory mechanisms underlying the specific changes in the CA gland necessary for the proper transition to adulthood. Support for this research was provided by the Spanish MINECO (grants BFU2009-10571 and CGL2014-55786-P to D.M. and CGL2012-36251 and CGL2015-64727-P to X.B.) and by the Catalan Government (2014 SGR 619 to D.M., J.L.M. and X.B.). The research has also benefited from FEDER funds. F.B.-C. is a recipient of a pre-doctoral research Grant from the MICINN. C.N. is a recipient of a postdoctoral research grant from CSIC. O.M. is recipient of a pre-doctoral research grant from Generalitat de Catalunya. |
| Databáze: | OpenAIRE |
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